Neglected OrthonairoViruses: Elucidating the vector-animal-human interface in Cameroon

被忽视的 Orthonairo 病毒：阐明喀麦隆的媒介-动物-人类界面

• 批准号: 461614762
• 负责人: Professor Dr. Martin Groschup
• 金额: --
• 依托单位: Faculté de Médecine et des Sciences Biomédicales
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/461614762?language=en
• 关键词: Neglected; OrthonairoViruses; Elucidating; vector; animal

Emerging diseases caused by arboviruses are of worldwide public health concern. However, our knowledge on the epidemiology, prevalence and distribution of many arboviral and particularly tick-borne infections in the sub-Saharan African region is limited. Besides Crimean-Congo haemorrhagic fever virus (CCHFV), considered the most impactful, tick-borne orthonairovirus, a number of other and putatively novel orthonairoviruses are known or at least suspected to circulate in Africa. However, their impact on veterinary and public health is understudied or entirely unknown.In this project, we aim to focus on the presence and distribution of known and potentially novel orthonairoviruses in Cameroon. To shed light on their veterinary and public health importance, we will combine elaborate field studies with laboratory-based experiments. Through sampling of vectors and incidental hosts including ruminants and humans, we aim to study the maintenance and potential transmission pathways of these tick-borne viruses at the vector-animal-human interface. Apart from serological and state-of-the-art molecular assays including virus sequencing and phylogenetic analyses, we intend to investigate the pathogenicity and zoonotic potential of known and novel orthonairovirus isolates using in-vitro and in-vivo animal experiments. Capacity building (personnel, laboratories, academic infrastructures) will be carried out by the German and two Cameroonian partners in close cooperation with two Cameroonian universities (Ngaoundere and Yaounde). Topics will cover novel diagnostic technologies (serology, qPCR, on-site next generation sequencing as well as biosafety measures.

由虫媒病毒引起的新发疾病是全球公共卫生关注的问题。然而，我们对撒哈拉以南非洲地区许多虫媒病毒，特别是蜱媒感染的流行病学、流行率和分布的了解有限。除了克里米亚-刚果出血热病毒（CCHFV）被认为是最具影响力的蜱传正鼻病毒外，已知或至少怀疑有许多其他新型正鼻病毒在非洲传播。然而，它们对兽医和公共卫生的影响是研究不足或完全unknowed.In这个项目中，我们的目标是集中在喀麦隆已知的和潜在的新的正鼻病毒的存在和分布。为了阐明它们在兽医和公共卫生方面的重要性，我们将把联合收割机精心设计的实地研究与实验室实验结合起来。通过对媒介和偶然宿主（包括反刍动物和人类）的采样，我们旨在研究这些蜱传病毒在媒介-动物-人类界面的维持和潜在传播途径。除了血清学和最先进的分子检测，包括病毒测序和系统发育分析，我们打算调查的致病性和人畜共患病的潜力，已知的和新的正鼻病毒分离株使用体外和体内动物实验。能力建设（人员、实验室、学术基础设施）将由德国和两个喀麦隆合作伙伴与两所喀麦隆大学（Ngaoundere和雅温得）密切合作进行。 主题将涵盖新的诊断技术（血清学，qPCR，现场下一代测序以及生物安全措施。