The epidemiological study on human adverse health effects 30 years after sprayed dioxins polluted environment in Vietnam

为了检测喷洒二恶英污染越南环境30多年来人体标本(包括母乳、脂肪组织和血清)中的二恶英水平及其与人体不良健康影响的关系，2002-2004年在越南中部两个村庄的越南居民中进行了四次检查。二恶英暴露区位于广三省的金镇公社，在那里喷洒除草剂(简称Quang Tri to Qt)，而河静省的金福公社(简称河静公社)被选为战时没有喷洒除草剂的控制区。这两个社区被北纬17度的非军事区分隔，这是越南战争中的军事边界。定量使用配备有HP-6980的高分辨率质谱仪(HRMS；JEOL mStation-JMS700)。越南除草剂暴露地区的人的乳汁、脂肪组织和血清中的二恶英水平显著高于二恶英非暴露地区的人。然而，在大多数样本中，TCDD对总I-TEQ的贡献不到20%。这些结果不同于以往的报道，即TCDD是橙剂毒性的有用指标，因为TCDD占据了二恶英的大部分。1，2，3，7，8-二氯二苯并二恶英和2，3，4，7，8-二氯二苯并呋喃在本研究的总TEQ中的贡献最大，有必要对其来源进行调查。两组的血清生化指标，如肝脏和甲状腺功能，无显著差异。母亲血清总PCDD/FS与胆碱酯酶呈显著负相关。经婴幼儿年龄调整后，乳汁中二恶英浓度与婴幼儿胸围呈显着正相关，但两地区婴幼儿生长发育无明显差异。

To examine dioxins levels in human specimen including breast milk, adipose tissue and serum and their relation with human adverse health effects over 30 years after sprayed dioxins polluted Vietnamese environment, Vietnamese inhabitants of two villages in central part of Vietnam were selected in four examinations between 2002 and 2004. Dioxin-exposed area is located in Cam Chinh commune in Quang Tri province where herbicides were sprayed (abbreviate Quang Tri to QT), while Cam Phuc commune in Ha Tinh province (abbreviate Ha Tinh to HT) is selected as control area where herbicides were not sprayed during the War. These two communes were separated by the demilitarized zone of latitude 17 degrees north line that was a military boundary in Vietnam War.Quantitation was performed by a high resolution mass spectrometer (HRMS ; JEOL MStation-JMS700) equipped with a gas chromatograph (HP-6980).Dioxin levels of human samples including breast milk, adipose tissue and serum in people in herbicide-exposed area in Vietnam is significantly higher than those in people in dioxin-unexposed control area. However, TCDD contributed less than 20% of the total I-TEQ in most samples. These results were different from the former reports that TCDD was a useful indicator for toxicity of Agent Orange since TCDD occupied most of dioxins. It is necessary to investigate the origin of 1,2,3,7,8-PCDD and 2,3,4,7,8-PCDF which were the most contributor in the total TEQ in this study.Biochemical indicators in sera such as liver or thyroid functions were not significantly different between two groups. Significant negative correlations were showed between Total PCDD/Fs and cholinesterase of mothers. Dioxin concentration in breast milk has significant association with chest measurement of infants after adjustment of age of infants although our results did not show difference between the growths of infants in two areas.