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Research on early detection of plant growth damage with ultraviolet rays and air pollution gases.

紫外线和空气污染气体对植物生长损伤的早期检测研究

基本信息

批准号：
14580576
负责人：
FUKUCHI Ken'ichi
金额：
$ 1.47万
依托单位：
Kisarazu National College of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580576/
关键词：
ultraviolet stress ozone stress fluorescence in plant leaves laser-induced fluorescence LIF spectra UV-LED 紫外線オゾン植物蛍光イメージング分光法蛍光の葉内分布 UV-B 植物診断

项目摘要

The influence of ultraviolet (UV) rays and air pollution gases exerted on plant growth was investigated by measurement of the UV laser excited LIF (Laser-induced fluorescence) spectra in peanuts (Arachis hypogaea L.) leaves. The following results were obtained.1)The value of R_<685/530>, fluorescence intensity ratio of 685 nm (F685) and 530 nm (F530), is proportional to both logarithm of ozone density C (ppm) and exposure time T(h) ; R_<685/530>=1-(0.346×lnT+0.958)×C.2)The value of R_<685/530> decreases in proportion to the UV-B fluence (Product of UV intensity I (Wm^<-2>) and exposure time T (h) ; R_<685/530>=1-0.0375×I×T.3)Changing of R_<740/685>, fluorescence intensity ratio of 740 nm (F740) and 685 nm (F685), is different in UV-B irradiation and ozone exposure.From the above-mentioned, it is understood that to detect early damage of UV stress and ozone stress is possible by the UV-LIF method.In order to measure all wavelength of the fluorescence distribution in leaf simultaneously, ISA (Imaging spectrogram analysis) device was newly developed. The following results were obtained by ISA measurements.4)The chlorophyll fluorescence ratio (F740/F685) increased according to the depth from the surface in control leaf, while it maintained a constant value in the UV-B damaged leaf.5)Blue fluorescence (400nm - 500nm) increased remarkably under UV-B irradiation in the upper epidermis of the leaf. It is thought that ISA device is useful for examining the growth condition of plant leaves.Moreover, the following became clear since the compound influence of the stress was evaluated based on the above-mentioned finding.6)UV-A irradiation to the control leaves usual hardly influences the revitalization decrease, while a remarkable damage is caused to the UV-B damaged leaves.

通过测定花生的紫外激光诱导荧光(LIF)光谱，研究了紫外线和大气污染气体对花生生长的影响。树叶。1)荧光强度比为685 nm(F685)和530 nm(F530)的R值与臭氧密度C(Ppm)和曝光时间T(H)的对数成正比；R&lt；685/530&gt；=1-(0.346×LNT+0.958)×C.2)R&lt；685/530&gt；)和暴露时间T(H)；R_&lt；685/530&gt；=1-0.0375×i×T。3)在UV-B辐射和臭氧照射下，荧光强度比740 nm(F740)和685 nm(F685)的荧光强度比不同。4)对照叶片的叶绿素荧光比值(F740/F685)随离地表深度的增加而增大，而UV-B损伤叶片的F740/F685保持恒定值。5)UV-B辐射下叶片上表皮的蓝色荧光(400 nm-500 nm)显著增强。在此基础上，对胁迫的复合影响进行了评价。6)UV-A辐射对对照叶片的影响一般不大，而UV-B辐射对受损叶片的伤害显著。