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Localization of Plasmon Field in Nanospace and Application to Fluorescence Analysis

纳米空间等离子体场的定位及其在荧光分析中的应用

基本信息

批准号：
15510111
负责人：
ISHIDA Akito
金额：
$ 1.34万
依托单位：
Kyoto Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15510111/
关键词：
localization of surface plasmon field nanospace fluorescence analysis fluorescence immuno assay fluorescence DNA assay microarray micro total analysis system surface plasmon enhanced fluorescence extraordinary light transmission 表面プラズモン

项目摘要

Localization of surface plasmon filed based on light-wavelength sized apertures on gold surface has been studied. The 500 nm and 600 nm apertures were prepared by projection method using polystyrene latex beads. ATR illumination of the gold films having the apertures resulted in extraordinary light transmission at the specific wavelengths corresponding to the aperture sizes. Fluorescence immunoassay in the apertures has been carried out by immobilization of anti-IgG on the bottom glass surface of the aperture. Upon putting a droplet of a PBS solution of IgG Texas Red conjugate during ATR illumination of the 20 apertures by 532 nm light induced remarkable increase in the fluorescence signal. The control experiment using BSA Texas Red conjugate gave no fluorescence signal, on the other hand. The fluorescence DNA assay in the apertures using 15mer oligonucleotides was also successful. The present results demonstrated fluorescence analyses in the nanospace based on plasmon enhanced excitation and development of the protein and DNA nanoarrays based on this novel localization method of photons in nanospace is expected.

研究了基于金表面光波长尺寸孔径的表面等离子体场局域化。用聚苯乙烯乳胶珠通过投影法制备500 nm和600 nm孔径。具有孔的金膜的ATR照射导致在对应于孔尺寸的特定波长处的异常光透射。通过将抗IgG固定在孔的底部玻璃表面上，在孔中进行荧光免疫测定。在ATR照射20个孔期间，通过532 nm光放置IgG德克萨斯红缀合物的PBS溶液的液滴，诱导荧光信号的显著增加。另一方面，使用BSA德克萨斯红缀合物的对照实验没有给出荧光信号。用15聚体寡核苷酸在孔中进行荧光DNA测定也是成功的。目前的研究结果表明，在纳米空间的荧光分析的基础上等离子体增强激发和蛋白质和DNA纳米阵列的发展基于这种新的定位方法的光子在纳米空间的预期。