Reproductive and population ecology of wild Arabidopsis

a.濒危的堪察加拟南芥亚种目前的分布。Kawasakiana进行了调查。对过去记录中所有可能的地点进行了调查，以确定目标植物的当前发生情况。我们确认存在3，1，1和1个种群分别在滋贺，三重，富山，高知县。B.对中华绒螯蟹的最大种群--琵琶湖种群的个体空间分布格局进行了研究。卡姆查蒂察湾川崎那。通过田间试验，包括去雄、套袋等处理，揭示了黑绒蒿的繁殖系统。卡姆查蒂察湾川崎那。事实证明，该物种主要通过自花授粉产生种子。该物种具有自授粉机制。d.我们使用生长室进行生长实验（其中一个是由本基金购买的Koitotoron HNM-S）。我们控制了温度和光周期，实验的目的是为了鉴定遗传变异。结果表明，南方居群比北方居群具有更强的春化要求。我们在所有研究的群体中都检测到了显著的遗传变异。e.我们在一个拟南芥群体中发现了毛状体的多态性。在兵库县中部的一个gemmifera。有毛和无毛的个体频率为1：1。我们发现突变位点内的一个序列的GL1，候选基因的毛损失，为个人没有毛。

a.Current distributions of endangered Arabidopsis kamchatca ssp. kawasakiana were investigated. All possible localities from the past records were investigated for the current occurrences of the target plant. We confirmed existences of 3,1,1, and 1 populations in Shiga, Mie, Toyama, and Kochi Prefectures, respectively. The species probably has extinct in Ehime and Aichi Prefectures.b.Spatial distribution patterns of individuals were investigated at Biwako population, the largest population of A. kamchatica ssp. kawasakiana. We recorded phenology and periods for germination and flowering in th natural population were determined.c.We conducted a set o field experiments including emasculation and bagging treatments to reveal breeding systems of A. kamchatica ssp. kawasakiana. It turned out that the species produces seeds by primarily by self polination. The species has a mechanisms of auto-polination.d.We conducted growth experiments using growth chambers (one of them is purchased by this grant, Koitotoron HNM-S). We controlled temperatures and photoperiods, and the experiments were designed to qualify genetic variation. Results indicated that southern populations have stronger requirements of vernalization for flowering compared with those of northern populations. We detected significant genetic variation for all of the studied populations.e.We discovered polymorphism on trichome presence in a population of Arabidopsis halleri sibsp. gemmifera in a central Hyogo. The frequencies of individuals with and without trichome was 1:1. We found mutation sites within a sequence of GL1, candidate gene for trichome loss, for the individuals without trichome.