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Behavioral and Histological study on the output resolution of orienting behavior of the frog.

青蛙定向行为输出分辨率的行为和组织学研究。

基本信息

批准号：
15570066
负责人：
NAKAGAWA Hideki
金额：
$ 2.24万
依托单位：
Kyushu Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570066/
关键词：
prey orienting behavior retrograde labeling distribution of cell bodies output resolution velocity control of orienting frog neuroethology functional unit 定位行動行動学視蓋出力細胞組織学

项目摘要

<Results from histological study>We examined spatial distribution of tectal efferent neurons related to prey orienting behavior in the frog Rana catesbeiana. Neurobiotin was injected as retrograde tracer into the nMLF and the anterodorsal nucleus in vitro preparations. Tangential sections were cut at 40 μm with a cryostat. Spatial autocorrelogram was computed to examine the regularity of the distribution of labeled cells. The results showed that 1)the labeled output neurons are classified into two groups (type A and B) based on their cell body size, 2)in each group, cell bodies cluster together in some sections and stand alone in others, however, all of the cell bodies show characteristic periodical distribution, 3)periodical distribution of type A neurons shows the same interval throughout the tectum, while that of type B neurons shows the region-specific intervals.<Results from behavioral study>We calculated the turn angle of the frog (θ1), the angle of the cricket before orientation response of the frog (θ2), the error in orientation response (θ1 minusθ2), the amount of time for orientation, and the angular velocity of orienting behavior. Angular velocity of frog's orienting turn showed the step-by-step increment occurring at 20,40,and 65 degrees. The peaks of the error in orientation were observed at the amplitudes where the step-by-step change of angular velocity occurred. When the frog comes to miss the prey object, the animal increases the turning velocity to capture the prey successfully. We also found that the frog showed an inaccurate turn with predetermined amplitude to the cricket which moves a long distance before orientation response. The amplitude showed good agreement with those where the step-by-step increment of turn velocity was observed. Therefore, for such a difficult target, they may turn into the nearest predetermined position to save energy.

组织学研究结果我们研究了与猎物定向行为有关的顶盖传出神经元在蛙体内的空间分布。将神经生物素作为逆行示踪剂注入NMLF和背侧前核。用冰冻恒温器在40μm处切开切面。计算空间自相关图以检查标记细胞的分布规律。结果表明：1)标记的输出神经元按胞体大小分为A型和B型两类；2)每组中，胞体在某些区段聚集在一起，在另一些区段是独立的，但所有胞体都呈典型的周期性分布；3)A型神经元在整个顶盖内的周期性分布是相同的，而B型神经元的周期性分布是区域特有的。我们计算了青蛙的转角(θ1)，青蛙定向反应前的蟋蟀角度(θ2)，定向反应的误差(θ1减去θ2)，定向的时间和定向行为的角速度。蛙体定向转向角速度在20度、40度、65度呈递增趋势。在角速度阶跃变化的幅值处观察到定向误差的峰值。当青蛙错过猎物时，动物会提高转身速度，成功地捕捉到猎物。我们还发现，蛙类对在定向反应之前移动了很长一段距离的蟋蟀表现出预定幅度的不准确转弯。其幅度与观察到转弯速度逐级递增的情况有很好的一致性。因此，对于如此困难的目标，他们可能会转向最近的预定位置来节省能源。