Analysis of the Hoxa3 gene that regulates the migration/differentiation of the cranial neural crest cells.

分析调节颅神经嵴细胞迁移/分化的 Hoxa3 基因。

• 批准号: 15570177
• 负责人: CHISAKA Osamu
• 金额: $ 2.37万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570177/
• 关键词: Hoxa3; Neural Crest; Placode; Glossopharyngeal Nerve; Pharyngeal Arch Artery; Parathyroid gland; 上鰓プラコード; アンチセンスモルフォリノオリゴ; 神経堤細胞; 鰓弓神経

In vertebrates, the proximal and distal sensory ganglia of the branchial nerves are derived from neural crest cells(NCCs) and placodes, respectively. We previously reported that in Hoxa3 knockout mouse embryos, NCCs and placode-derived cells of the glossopharyngeal nerve were defective in their migration. In this report, to address the question as to in which cell type is the expression of Hoxa3 responsible for the above phenotype, we blocked the expression of the gene with anti-sense morpholino oligonucleotides(MO) specifically in either NCCs/the neural tube or placodal cells of chicken embryos. Our results showed that Hoxa3 function is required for the migration of the epibranchial placode-derived cells and that Hoxa3 regulate these cell migration in both NCCs/the neural tube and placodal cells. We also found that chicken and mouse Hoxa3 are expressed in the anterior head, which had been originally thought to be a Hox-negative region.Also, in Hoxa3 mutant mice have defects in the pharyngeal arch artery and the parathyroid gland organogenesis. We have checked the migration and differentiation of the neural crest in this area. Our results indicate that the initial migration of the neural crest is normal but further proliferation and/or differentiation of the crest cells are affected in the Hoxa3 mutant mouse embryos.

在脊椎动物中，鳃神经的近端和远端感觉神经节分别来源于神经嵴细胞和基板。我们以前报道过，在Hoxa 3基因敲除小鼠胚胎中，舌咽神经的NCC和基板来源的细胞在迁移中有缺陷。在这份报告中，为了解决的问题，在哪种细胞类型是负责上述表型的Hoxa 3的表达，我们阻止了基因的表达与反义吗啉代寡核苷酸（MO），特别是在任何NCC/神经管或基板细胞的鸡胚。我们的研究结果表明，Hoxa 3功能所需的上鳃基板衍生细胞的迁移和Hoxa 3调节这些细胞迁移在NCC/神经管和基板细胞。我们还发现，Hoxa 3基因在鸡和小鼠的前头部表达，而这一区域原本被认为是Hox阴性区域，而且Hoxa 3基因突变小鼠的咽弓动脉和甲状旁腺器官发生都有缺陷。我们已经检查了该区域神经嵴的迁移和分化。我们的研究结果表明，最初的迁移的神经嵴是正常的，但进一步的增殖和/或分化的嵴细胞的影响，在Hoxa 3突变小鼠胚胎。

项目成果

The role of Hoxa3 gene in parathyroid gland organogenesis of the mouse

• DOI: 10.1177/002215540405200508
• 发表时间: 2004-05-01
• 期刊: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
• 影响因子: 3.2
• 作者: Kameda, Y;Arai, Y;Chisaka, O
• 通讯作者: Chisaka, O

Hoxa3 regulates the proliferation and differentiation of the third pharyngeal arch mesenchyme in mice

• DOI: 10.1007/s00441-004-1042-z
• 发表时间: 2005-04-01
• 期刊: CELL AND TISSUE RESEARCH
• 影响因子: 3.6
• 作者: Chisaka, O;Kameda, Y
• 通讯作者: Kameda, Y