Research on Genetic Diversity of Sika Deer living in Osaka

据认为，1970年代大阪府梅花鹿被分成三个种群，与周围环境隔离。有三个种群（鼻子，米努和高摫）。据推测，最近有3个种群扩大了分布范围。对3个群体的DNA进行PCR扩增，根据电泳条带的遗传多态性选择微卫星引物。选取5条引物：BM2934、BoviRBP、MB009、OarFCB193、TGLA53。假设各波段的出现为自主变量，采用Ward方法进行聚类分析。利用亲属关系软件计算个体间的遗传亲缘关系。通过聚类分析，将人群分为Nose组、Minoo组和Takatsuki组。Nose、Minoo和Takatsuki的遗传亲缘度分别为0.45、0.42和0.51。以鼻、米诺、高月、兵库县、京都、和歌山和白尾鹿为研究对象确定线粒体DNA d环区排列。利用计算机软件Clustal W，利用除串联重复区外的D-loop序列，绘制系统发育树。Minoo和Takatsuki的个体分别组成了这个集群。此外，利用DDBJ/EMBL/GeneBank核苷酸阵列数据库的登录号AB012364-AB012385数据构建系统发育树。在聚类分析中，大阪的所有个体都被归为日本北部群体。通过D-loop域的单倍型计算遗传多样性。Nose、Minoo和Takatsuki的遗传多样性分别为0.64、0.67和0.50。

It is thought that sika deer in Osaka Prefecture were divided into three populations in 1970's, and were isolated from the surrounding. There have been three populations (Nose, Minoo and Takatsuki). Recently, three populations were presumed to have expanded the distribution region. DNA samples from three populations were amplified by PCR and primers of microsatellites were seleced according to genetic polymorphism seen in the appearance of the bands of electrophoresis. Five primers were selected: BM2934,BoviRBP, MB009,OarFCB193,TGLA53.The appearance of each band was assumed as autonomous variable, and the cluster analysis by the Ward method was executed.Moreover, the genetic relatedness between individuals was calculated by software Kinship. As a result of the cluster analysis, the populations divided into two groups, Nose group, and Minoo and Takatsuki group. The genetic relatedness of Nose, Minoo and Takatsuki was 0.45(mean), 0.42 and 0.51 respectively. The array of the D-loop area of mitochondria DNA was decided by using as Nose, Minoo, Takatsuki, Hyogo, Kyoto, Wakayama and White-tailed deer. The phylogenetic tree was made with computer software Clustal W by using arrays of D-loop except the tandem-repeat region. The individuals of Minoo and Takatsuki formed the cluster respectively. In addition, the phylogenetic tree was made by data of Accession Number AB012364-AB012385 of the DDBJ/EMBL/GeneBank nucleotide array data base.In the cluster analysis, all the individuasl of Osaka were classified as northem Japan group. A genetic diversity was caliculated by D-loop dmain's Haplotype. A genetic diversity was 0.64 in Nose, 0.67 in Minoo, and 0.50 in Takatsuki, respectively.