Analysis of telomere metabolism in Werner's syndrome patients.
维尔纳综合征患者端粒代谢分析。
基本信息
- 批准号:15590328
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We collected tissue samples from 7 different patients with Werner syndrome, two of whom provided samples on two different dates, giving a total of 9 samples. In these and control tissues, telomere length was measured by Southern blot analysis after digestion by Hinf I and Rsa I. Telomerase activity was examined by the TRAP assay and ISH. Telomere lengths measured using Hinf I and Rsa I were well correlated. Mean telomere length in the epidermis of 8 normal neonates was 13.3 kbp and the rate of reduction of telomere length for the epidermis was 36 bp/y. In the patients with Werner syndrome, the yearly rate of reduction of telomere length was 161 bp, which was estimated to be about 5 times the reduction rate for normal epidermis. In the 2 Werner patients who provided tissue samples on different dates, the yearly rates of reduction of telomere length in the tissues obtained were also higher than those for corresponding samples from controls. Telomere lengths were 7.0-12.8 kbp (39-61 y) for striated muscle fibers, 8.5 kbp (61 y) for bone marrow, 10.8 kbp (61 y) for cartilage, 5.6 kbp (61 y) for marginal epidermis surrounding a skin ulcer, and 11.5 kbp (41 y) for fibroblasts. Telomerase activity was present in 8% of epidermal samples from normal controls and in one sample of epidermis from Werner syndrome. hTERT mRNA expression was observed in epidermal basal cells, endothelial cells, and lymphocytes. We have been able to maintain 2 strains of epidermis and fibroblasts in culture. Based on our results, we have demonstrated for the first time that telomere shortening is more rapid in patients with Werner syndrome than in normal individuals. We have also successfully devised an original method for measuring telomere length in routinely prepared tissue sections by FISH.
我们收集了7名不同的Werner综合征患者的组织样本,其中两名患者在两个不同的日期提供了样本,总共提供了9个样本。在这些组织和对照组织中,用Hinf I和Rsa I消化后,用Southern杂交分析端粒长度,用TRAP法和ISH检测端粒酶活性。用Hinf I和Rsa I测量的端粒长度有很好的相关性。8例正常新生儿表皮端粒平均长度为13.3kBP,端粒缩短速率为36BP/年,Werner综合征患儿端粒长度年缩短速率为161bp,约为正常皮肤端粒缩短速率的5倍。在2例在不同日期提供组织标本的Werner患者中,获得的组织端粒长度的年减少率也高于相应的对照组样本。横纹肌纤维端粒长度为7.0~12.8KBP(39~61年),骨髓为8.5KBP(61年),软骨为10.8KBP(61年),皮肤溃疡周围边缘表皮为5.6KBP(61年),成纤维细胞为11.5KBP(41年)。端粒酶活性在8%的正常人和1例Werner综合征患者的表皮中均有表达。HTERT基因在表皮基底层细胞、内皮细胞和淋巴细胞中均有表达。我们已经能够在培养中保持2株表皮和成纤维细胞。基于我们的结果,我们首次证明了Werner综合征患者的端粒缩短速度比正常人更快。我们还成功地设计了一种独创的方法,用于测量常规FISH组织切片中的端粒长度。
项目成果
期刊论文数量(54)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Clinical application of human telomerase reverse transcriptase gene expression in thyroid follicular tumors by fine-needle aspirations using in situ hybridization.
原位杂交细针穿刺检测人端粒酶逆转录酶基因在甲状腺滤泡性肿瘤中的临床应用。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:Kammori M;et al.
- 通讯作者:et al.
Arai T., Esaki Y., Sawabe M., Honma N., Takubo K.: "Hypermethylation of the hMLH1 promoter with absent hMLH1 expression in medullary-type poorly differentiated colorectal adenocarcinoma in the elderly."Mod.Pathol.. 17(2). 172-179 (2004)
Arai T.、Esaki Y.、Sawabe M.、Honma N.、Takubo K.:“老年人髓质型低分化结直肠腺癌中 hMLH1 启动子的高甲基化且缺乏 hMLH1 表达。”Mod.Pathol.. 17(
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kammori M., 他4人, Takubo K.: "Clinical application of human telomerase reverse trnscriptase gene expression in thyroid follicular tumors by fine - needle aspirations using in situ hybridization."Int.J.Oncol.. 22(3). 985-991 (2003)
Kammori M.,其他 4 人,Takubo K.:“通过原位杂交细针穿刺检测人端粒酶逆转录酶基因表达在甲状腺滤泡肿瘤中的临床应用。”Int.J.Oncol.. 22(3) -。 991(2003)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
田久保海誉他: "幕内雅敏監修、幕内博康編、Knack & Pitfallsシリーズ「食道外科の要点と盲点」(分担出筆)Barrett食道の生検病理診断"文光堂(東京). (2003)
Kaiho Takubo 等:“幕内正敏监督、幕内博康编辑,Knack & Pitfalls 系列“食管手术的要点和盲点”(合着)Barrett 食管的活检病理诊断”文科堂(东京)(2003 年)。 )
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
TAKUBO Kaiyo其他文献
TAKUBO Kaiyo的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('TAKUBO Kaiyo', 18)}}的其他基金
Chimerism of human transplanted liver and possibility of anti-aging effect of chimera hepatocytes
人移植肝嵌合现象及嵌合肝细胞抗衰老作用的可能性
- 批准号:
23659193 - 财政年份:2011
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Telomere dysfunction and chromosomal instability in uninvolved tissue with carcinoma by original FISH
原始 FISH 检测癌未受累组织的端粒功能障碍和染色体不稳定性
- 批准号:
21390109 - 财政年份:2009
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (B)