Role of class II MHC bound antigenic peptide in the pathogenesis of inflammatory bowel disease.

II 类 MHC 结合抗原肽在炎症性肠病发病机制中的作用。

• 批准号: 15590676
• 负责人: OSHITANI Nobuhide
• 金额: $ 1.98万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590676/
• 关键词: Inflammatory bowel disease; Crohn's disease; ulcerative colitis; class II MHC; antigenic peptide; tandem mass analysis; amino acid sequence; 炎症性腸疾患; HLA-DR; 抗ペプチド; HLA-DP; 免疫吸着カラム

Isolation of antigenic peptides from the MHC-groove has contributed to the understanding of T cell immune responses. We already reported antigenic peptides isolated from human intestinal HLA-DR grove contained various enteral antigens.However, naturally bound peptide ligands for HLA-DP have yet to be reported in clinical samples. We examined antigenic peptides bound to the HLA-DP as well as HLR-DR groove in human intestine by ion-trap tandem mass spectrometry equipped with online reverse-phase high performance liquid chromatography. We detected 7 HLA-DR associated peptides and 10 HLA-DP associated peptides from 4 controls, 4 patients with ulcerative colitis, and 9 patients with Crohn's disease. The calculated molecular masses (m/z) of these peptides reangesd from 1430.7 to 2987.3 and 1212.6 to 2105.0 representing 15 to 26 and 15 to 18 amino-acid residues from HLA-DR and HLA-DP associated peptides, respectively. Most of these parent proteins were exogenous proteins including Escherichia coli, and Carnorhabditis elegans. The present results suggest that in vivo antigen processing by antigen-presenting cells and T lymphocytes in human intestine are participated with exogenous antigen presentation.

从 MHC 沟中分离抗原肽有助于了解 T 细胞免疫反应。我们已经报道了从人肠HLA-DR丛中分离出的抗原肽含有多种肠内抗原。然而，HLA-DP的天然结合肽配体尚未在临床样品中报道。我们通过配备在线反相高效液相色谱的离子阱串联质谱法检查了与人肠中的 HLA-DP 和 HLR-DR 沟结合的抗原肽。我们从 4 名对照者、4 名溃疡性结肠炎患者和 9 名克罗恩病患者中检测到 7 种 HLA-DR 相关肽和 10 种 HLA-DP 相关肽。这些肽的计算分子量(m/z)从1430.7到2987.3和1212.6到2105.0重新调整，分别代表来自HLA-DR和HLA-DP相关肽的15到26和15到18个氨基酸残基。这些亲本蛋白大多数是外源蛋白，包括大肠杆菌和秀丽隐杆线虫。目前的结果表明，人肠道中抗原呈递细胞和T淋巴细胞的体内抗原加工参与了外源抗原呈递。

项目成果

Sawa Y, Oshitani N et al.: "Comprehensive analysis of intestinal cytokine messenger RNA profile by real-time quantitative polymerase chain reaction in patients with inflammatory bowel disease"Int J Mol Med. 11. 175-179 (2003)

Sawa Y、Oshitani N 等人：“通过实时定量聚合酶链反应对炎症性肠病患者肠道细胞因子信使 RNA 谱进行综合分析”Int J Mol Med。

Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitlis.

溃疡性结肠炎患者阑尾中未成熟浆细胞增殖增加的意义。

• 发表时间: 2005
• 期刊: Int J Mol Med 15
• 作者: Kawachiya T;Oshitani N;Jinno Y;Watanabe K;Nakamura S;Fujiwara Y;Higuchi K;Maeda K;Nishiguchi Y;Hirakawa K;Matsumoto T;Arakawa T.
• 通讯作者: Arakawa T.

Accumulation of mitochondrial DNA mutation with colorectal carcinogenesis in ulcerative colitis.

• DOI: 10.1038/sj.bjc.6602664
• 发表时间: 2005-08-08
• 期刊: British journal of cancer
• 影响因子: 8.8

Mycobacterial trehalose 6,6′-dimycolate preferentially induces type 1 helper T cell responses through signal transducer and activator of transcription 4 protein

• DOI: 10.1016/j.micpath.2005.03.003
• 发表时间: 2005-07-01
• 期刊: MICROBIAL PATHOGENESIS
• 影响因子: 3.8
• 作者: Oiso, R;Fujiwara, N;Kobayashi, K
• 通讯作者: Kobayashi, K

Oshitani N et al.: "Analysis of intestinal HLA-DR bound peptides and dysregulated immunre responses to enteric flora in the pathogenesis of inflammatory bowel disease"Int J Mol Med. 11. 99-104 (2003)

Oshitani N 等人：“炎症性肠病发病机制中肠道 HLA-DR 结合肽和肠道菌群免疫反应失调的分析”Int J Mol Med。