The study on the mechanism of production of autoantibodies in autoimmune hemolytic anemia.

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Rh polypeptides, especially RhCcEe, are the common autoantigens in autoimmune hemolytic anemia (AIHA). Rh blood group antigens and the autoantigens in AIHA are conformational antigens and constructed by six extracellular loops of Rh polypeptides. Identification of replica of autoantigen epitopes (mimotope) was performed by means of screening phage displayed random peptide library.1.Identification of Rh mimotopes, reacting monoclonal anti-D antibody and/or anti-E antibody.2.Identification of autoantigen mimotopes, reacting autoantibody from AIHA patients.3.Six out of eight of the autoantibodies reacted the Rh mimotopes, therefore it is assumed that the Rh mimotopes might be included into autoantigen mimotopes.4.These mimotopes will make it possible to identify and categorize autoantibodies in AIHA, to map epitopes of autoantigens on Rh peptides and to verify molecular mimicry between foreign antigens and Rh polypeptides as the etiology of autoantibodies in AIHA.