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The study on the mechanism of production of autoantibodies in autoimmune hemolytic anemia.

自身免疫性溶血性贫血自身抗体产生机制的研究。

基本信息

批准号：
15591018
负责人：
KAMESAKI Toyomi
金额：
$ 2.18万
依托单位：
Jichi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591018/
关键词：
autoimmune hemolytic anemia Rh blood groups mimotope autoantibody random peptide library band 3 linear peptide molecular mimicry ミモトープ Coombs陰性自己免疫性溶血性貧血 CD47 交叉反応

项目摘要

Rh polypeptides, especially RhCcEe, are the common autoantigens in autoimmune hemolytic anemia (AIHA). Rh blood group antigens and the autoantigens in AIHA are conformational antigens and constructed by six extracellular loops of Rh polypeptides. Identification of replica of autoantigen epitopes (mimotope) was performed by means of screening phage displayed random peptide library.1.Identification of Rh mimotopes, reacting monoclonal anti-D antibody and/or anti-E antibody.2.Identification of autoantigen mimotopes, reacting autoantibody from AIHA patients.3.Six out of eight of the autoantibodies reacted the Rh mimotopes, therefore it is assumed that the Rh mimotopes might be included into autoantigen mimotopes.4.These mimotopes will make it possible to identify and categorize autoantibodies in AIHA, to map epitopes of autoantigens on Rh peptides and to verify molecular mimicry between foreign antigens and Rh polypeptides as the etiology of autoantibodies in AIHA.

Rh多肽，尤其是RhCcEe，是自身免疫性溶血性贫血（AIHA）中常见的自身抗原。Rh血型抗原和AIHA中的自身抗原是构象抗原，由Rh多肽的六个胞外环构成。自身抗原表位复制品的鉴定用噬菌体展示随机肽库筛选Rh抗原模拟表位，1. Rh抗原模拟表位的鉴定，与抗-D和/或抗-E单克隆抗体反应; 2.自身抗原模拟表位的鉴定，与AIHA患者自身抗体反应; 3. 8株自身抗体中有6株与Rh抗原模拟表位反应，因此推测Rh模拟表位可能包括在自身抗原模拟表位中。4.这些模拟表位将使AIHA中自身抗体的鉴定和分类成为可能，目的：定位Rh多肽上自身抗原的表位，并验证外源抗原与Rh多肽之间的分子模拟作为AIHA中自身抗体的病因。