Multi-Photon Laser Scanning Microscope

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Inflammatory processes are associated with a broad variety of conditions such as infections, cancer metastasis, thrombosis and ischemic diseases. However, understanding of the underlying pathophysiology has remained incomplete. Detailed elucidation of innate and adaptive immune responses in vivo, specifically immune cell recruitment, migration, activation and their functions within distinct niches and organ environments, is of pivotal importance for the development of novel therapeutic strategies to resolve tissue inflammation. Multiphoton intravital microscopy has revolutionized high-resolution imaging allowing researchers to gain profound insight into inflammatory processes within tissues and organ systems in vivo. This is achieved by enabling real-time and long-term high-resolution deep tissue imaging, while exerting only minimal phototoxicity. The use of multiphoton intravital microscopy in combination with transgenic mouse models represents a cornerstone of our research into cancer metastasis, thrombosis and immune cell trafficking and functions in sterile inflammation and infections.All research projects outlined in this proposal rely on multiphoton intravital microscopy to achieve their aims. As we work with live animals, fast deep tissue imaging with low photo-toxicity is a critical issue. The proposed acquisition of an advanced multiphoton microscope would provide state-of-the-art functionality including high spatio-temporal resolution combined with high flexibility in scanning strategies, such as sensitivity and speed. Our current multiphoton intravital microscope device is 12 years old and running at maximum capacity. However, the system lacks sensitivity, speed, resolution, laser excitation capabilities, as well as a motorized stage. Together, the current system does not provide state-of-the-art imaging capabilities in terms of speed and resolution but also prohibits expansion of boundaries in the study of immunobiology, for example through in vivo photo-manipulation. The acquisition of a new integrated multiphoton system will allow our research projects to stay competitive and carry out cutting edge scientific research.