Multi-Photon Laser Scanning Microscope

多光子激光扫描显微镜

• 批准号: 464511686
• 金额: --
• 依托单位: Ludwig-Maximilians-Universität München (LMU)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/464511686?language=en
• 关键词: Multi; Photon; Laser; Scanning; Microscope

Inflammatory processes are associated with a broad variety of conditions such as infections, cancer metastasis, thrombosis and ischemic diseases. However, understanding of the underlying pathophysiology has remained incomplete. Detailed elucidation of innate and adaptive immune responses in vivo, specifically immune cell recruitment, migration, activation and their functions within distinct niches and organ environments, is of pivotal importance for the development of novel therapeutic strategies to resolve tissue inflammation. Multiphoton intravital microscopy has revolutionized high-resolution imaging allowing researchers to gain profound insight into inflammatory processes within tissues and organ systems in vivo. This is achieved by enabling real-time and long-term high-resolution deep tissue imaging, while exerting only minimal phototoxicity. The use of multiphoton intravital microscopy in combination with transgenic mouse models represents a cornerstone of our research into cancer metastasis, thrombosis and immune cell trafficking and functions in sterile inflammation and infections.All research projects outlined in this proposal rely on multiphoton intravital microscopy to achieve their aims. As we work with live animals, fast deep tissue imaging with low photo-toxicity is a critical issue. The proposed acquisition of an advanced multiphoton microscope would provide state-of-the-art functionality including high spatio-temporal resolution combined with high flexibility in scanning strategies, such as sensitivity and speed. Our current multiphoton intravital microscope device is 12 years old and running at maximum capacity. However, the system lacks sensitivity, speed, resolution, laser excitation capabilities, as well as a motorized stage. Together, the current system does not provide state-of-the-art imaging capabilities in terms of speed and resolution but also prohibits expansion of boundaries in the study of immunobiology, for example through in vivo photo-manipulation. The acquisition of a new integrated multiphoton system will allow our research projects to stay competitive and carry out cutting edge scientific research.

炎症过程与多种病症有关，例如感染、癌症转移、血栓形成和缺血性疾病。然而，对潜在病理生理学的理解仍然不完整。详细阐明体内先天性和适应性免疫反应，特别是免疫细胞的招募、迁移、激活及其在不同生态位和器官环境中的功能，对于开发解决组织炎症的新治疗策略至关重要。多光子活体显微镜彻底改变了高分辨率成像，使研究人员能够深入了解体内组织和器官系统内的炎症过程。这是通过实现实时和长期高分辨率深层组织成像，同时仅产生最小的光毒性来实现的。多光子活体显微镜与转基因小鼠模型的结合使用是我们研究癌症转移、血栓形成和免疫细胞运输以及无菌炎症和感染中的功能的基石。本提案中概述的所有研究项目都依靠多光子活体显微镜来实现其目标。当我们使用活体动物时，具有低光毒性的快速深层组织成像是一个关键问题。拟议购买的先进多光子显微镜将提供最先进的功能，包括高时空分辨率以及扫描策略的高灵活性，例如灵敏度和速度。我们目前的多光子活体显微镜设备已有 12 年历史，并且以最大容量运行。然而，该系统缺乏灵敏度、速度、分辨率、激光激发能力以及电动平台。总之，当前系统在速度和分辨率方面不提供最先进的成像能力，但也禁止扩展免疫生物学研究的边界，例如通过体内照片操纵。获得新的集成多光子系统将使我们的研究项目保持竞争力并开展尖端科学研究。