Proliferation of human lymphoid malignancy by the Interaction of follicular dendritic cells

生发中心B细胞在滤泡树突状细胞(FDC)的帮助下分化，滤泡性淋巴瘤(FL)也含有大量FDC。为了阐明FL与FDC之间的相互作用，将FDC样细胞系HK与FL共培养。HK不能充分支持FL细胞的生长，但在IL-2、IL-4、IL-7和IL-15的混合物存在下，HK仅支持FL中存在的CD4()T细胞的生长。一个月后，细胞数量急剧增加，增加了1000多倍。这些数据表明，在IL-2、IL-4、IL-7和IL-15存在的情况下，HK细胞支持CD4()T细胞的生长。3例成人T细胞白血病/淋巴瘤(ATLL)的淋巴结分别与HK和IL-2、4、7、15共培养3个月以上，以确定HK细胞是否刺激了T细胞瘤的生长。三种细胞中的一种是通过T细胞受体γ链重排模式从原始淋巴瘤细胞分化而来的。这个ATLL来源的细胞系依赖于HK，因为没有HK，它在几周后停止增殖，最终死亡。提示部分成人T细胞白血病/淋巴瘤FDC在与T细胞相互作用过程中为其生长提供了良好的环境。为了确定其他T细胞淋巴瘤是否被HK刺激，将一些T细胞淋巴瘤细胞与HK细胞共培养。其中，2/3的血管免疫母细胞性T细胞淋巴瘤在IL-2、IL-4、IL-7和IL-15存在的情况下也在HK细胞上增殖。原始淋巴瘤细胞的克隆带在生长细胞中是保守的。这些数据表明，在淋巴结中，FDCs为几种类型的恶性淋巴瘤提供了良好的增殖环境。

Germinal center B cells are differentiated with the help of follicular dendritic cells(FDC), and follicular lymphoma(FL) also contain substantial FDC. To clarify the interaction between FL and FDC, FDC like cell line HK was co-cultured with FL. HK did not support the growth of FL cells sufficiently, however, in the presence of the mixture of IL-2, IL-4, IL-7, and IL-15,HK supported exclusively the growth of CD4(+) T cells which existed in FL. After a month, cell number highly increased more than 1000 folds. These data indicated that HK cells supported the growth of CD4(+) T cells in the presence of IL-2, IL-4, IL-7 and IL-15. Then, to confirm whether HK cells stimulate the growth of CD4(+) T-lymphoma or not, lymph node of three Adult T-cell leukemia/lymphoma(ATLL) were co-cultured with HK and IL-2,4,7,15. In all three cases, CD4(+) T-cells proliferate vigorously more than 3 months. One of three cells was derived from original lymphoma cells by using T-cell receptor gamma chain rearrangement pattern. This ATLL derived cell line is dependent on HK, because without HK, it stopped to proliferate a few weeks later and finally died. These data suggested that some of Adult T-cell leukemia/lymphoma, FDC provide the favorable environment for its growth in the course of the interaction of FDC and T-cells. In order to confirm whether other T-cell lymphomas are stimulated by HK or not, some T-cell lymphoma cells were co-cultured with HK cells. Among them, two of three angioimmunoblastic T-cell lymphoma also proliferated on the HK cells in the presence of IL-2, IL-4, IL-7 and IL-15. The clonal band of the original lymphoma cells was conserved in the growing cells. These data indicated in the lymph node, FDCs afford favorable environment to proliferate several types of malignant lymphoma.