Detection and elimination of resting spores of Olpidium vectoring lettuce big-vein disease in lettuce seedling nursery production

本项目将设计莴苣大静脉病（LBVD）病媒油菜油斑菌（Olpidium brassicae）的核酸和血清学检测方法，用于苗圃环境下莴苣育苗各阶段的检测。这将是第一个开发可持续和对环境负责的苗圃最佳实践方案的研究，用于在常规幼苗生产和生菜产业管理中对芸苔芸苔的综合管理。这些协议将确保防止LBVD从受污染的生菜苗圃传播到农场。它还将是在具有不同土壤和农业做法的新土地上建立一种疾病的独特研究。

This project will devise nucleic acid and serology methods for detection of Olpidium brassicae, vector of lettuce big-vein disease (LBVD), during various phases of lettuce seedling production in nursery environments. It will be the first study to develop sustainable and environmentally responsible nursery best-practice protocols for integrated management of O. brassicae in routine seedling production and for management within the lettuce industry. These protocols will ensure that the spread of LBVD from contaminated lettuce nurseries to farms is prevented. It will also be a unique study of the establishment of a disease in new land with different soils and agricultural practices.