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FUNCTIONAL ANALYSIS OF PLAKIN FAMILY PROTEINS

Plakin 家族蛋白的功能分析

基本信息

批准号：
15591203
负责人：
KARASHIMA Tadashi
金额：
$ 2.18万
依托单位：
KURUME UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591203/
关键词：
KERATINOCYTE PLAKIN FAMILY TRANSGLUTAMINASE

项目摘要

Plakin family proteins have aroleascytolinker proteins, which includes desmoplakin, plectin, Bullous pemphigoid antigen 1(BPAG1), envoplakin, periplakin andepiplakin They have an amino-terminal globular domain, acentrarod domain and a carboxy-terminalglobular domain The an amino-terminal globular domain mediating the interaction with the plasma membrane and the carboxy-terminalglobular domain associating with intermediate filaments. We find that the envoplakin stably transfected mouse keratinocytes showed remarkable keratinisation/differentiation in culture system. This led us to propose that the plakin family proteins can mediates keratinisation/differentiation of keratinocyte. The aim of our experiments was to carryout amoredetailed study about the relationship of plakin family proteins and epidermal keratinocyte Keratinisation/differentiation.Results : 1.Envoplakin/Periplakin stably transfected mouse keratinocytes showed remarkable keratinisation/differentiation in 3-D keratinocyte culture system. About the other plakins, series of the experiment is now on the way.2.In the epidermal malignant tumors, the localization of the plakin family proteins and in situ activity of transglutaminase were remarkably disturbed, but in the benign tumors were not.

Plakin家族蛋白包括桥粒斑蛋白（desmoplakin）、网蛋白（plectin）、大疱性类天疱疮抗原1（BPAG 1）、包斑蛋白（envoplakin）、周斑蛋白（periplakin）和斑片蛋白（depipplakin）。我们发现，稳定转染envoplakin的小鼠角质形成细胞在培养系统中显示出显着的角化/分化。这使我们提出斑蛋白家族蛋白可以介导角质形成细胞的角质化/分化。结果：1.稳定转染Envoplakin/Periplakin基因的小鼠角质形成细胞在三维培养体系中表现出明显的角质形成/分化。2.在表皮恶性肿瘤中，plakin家族蛋白的定位和转氨酶的原位活性受到明显干扰，而在良性肿瘤中，plakin家族蛋白的定位和转氨酶的原位活性没有受到明显干扰。