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Correlation between cellular expression of transcription factors and of phosphorylated glutamate receptors under conditioned fear stress

条件性恐惧应激下转录因子和磷酸化谷氨酸受体的细胞表达之间的相关性

基本信息

批准号：
15591234
负责人：
HASHIGUCHI Hiroyuki
金额：
$ 2.24万
依托单位：
Faculty of Medicine, University of Miyazaki (2004)宮崎医科大学 (2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

we examined the effects of conditioned fear stress(CF) as a stressful manipulation on Fos expression in both monoaminergic, Y-aminobutyric acid(GABA)-ergic and glutamatergic cells in the the prefrontal cortex(PFC), cingulate cortex(Cg), nucleus accumbens(NAC), amygdala(AMY), ventral tegmental area(VTA), substantia nigra(SN) dorsal raphe nucleus(DR), and locus coeruleus(LC). With a double-labeling technique, Fos immunostaining was combined with that for tyrosine hydroxylase(TH), serotonin (5-HT) or GABA for histochemical identification of activated neurons following the stress.1.Following both the foot shock(FS) and CF stress, the number of Fos-positive cells significantly increased in many regions including the PFC, dorsal DR, and LC whereas did not increase in NAC, AMY, VTA,and SN. In the CF group, Fos-like immunoreactivity was lower in PFC,DR, or LC than corresponding value in the FS group.2.Sixty percentages of Fos-positive cells in the LC were colocalized with either TH or GABA. Similarly, many of Fos-positive cells in the DR were colocalized with either 5-HT or GABA.3.Forty-Fifty percentages of Fos-positive cells in the PFC, PVN, DR, LC were colocalized with glutamate N-methyl-D-aspartate(NMDA) receptor subunit 1(NR-1).4.Following both the FS and CF stress, Protein of the phosphorylated glutamate receptor subunits did not change in the PFC, PVN, SN, DR, LC.These results indicate that the CF could induce intense Fos expression in the noradrenergic and serotonergic neurons, but not in the dopaminergic neurons, and that GABAergic and glutamatergic neurons in these nuclei are commonly activated by the stress.

我们观察了条件性恐惧应激(CF)对前额叶皮质(PFC)、扣带回(CG)、伏隔核(NAC)、杏仁核(AMY)、腹侧被盖区(VTA)、黑质(SN)、中缝背核(DR)和蓝斑(LC)单胺能、Y-氨基丁酸(GABA)能和谷氨酸能神经元Fos表达的影响。Fos免疫组织化学染色结合酪氨酸羟化酶(TH)、5-羟色胺(5-HT)或GABA免疫组织化学方法对应激后激活的神经元进行鉴定。1.足底电击(FS)和CF应激后，PFC、背侧DR和LC等部位的Fos阳性细胞数显著增加，而NAC、AMY、VTA和SN的Fos阳性细胞数无明显增加。在CF组，Fos阳性细胞在PFC、DR或LC中均低于FS组。2.LC中60%的Fos阳性细胞与TH或GABA共定位。PFC、PVN、DR、LC中45%的Fos阳性细胞与谷氨酸N-甲基-D-天冬氨酸(NMDA)受体亚单位1(NR-1)共定位。4.在FS和CF胁迫下，PFC、PVN、SN、DR、LC中磷酸化谷氨酸受体亚单位的蛋白表达没有改变。结果表明，CF可诱导去甲肾上腺素能神经元和5-羟色胺能神经元表达Fos，但在多巴胺能神经元中不表达Fos这些核团中的GABA能和谷氨酸能神经元通常被压力激活。