The effect of gefinitb (Iressa) on radiation of lung-analysis of anti-cancer effect and radiation pneumonia

吉非替尼（易瑞沙）对肺部辐射的影响——抗癌作用及放射性肺炎分析

• 批准号: 15591262
• 负责人: ONISHI Hiroshi
• 金额: $ 2.37万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591262/
• 关键词: rat; radiation pneumonitis; gefinitib; cisplatin; lung fibrosis; blood count and chemistry; histopathology; respiratory function; 放射線肺障害; 化学放射線療法; 間質性肺炎; 実験モデル; 抑制効果判定; 電子線照射; 放射線; 抗腫瘍効果; 増強効果; ラット放射線肺炎モデル

We conducted the respiratory function determination, pulmonary function determination, blood biochemistry and histological study in irradiated rats, and studied the effect of irradiation on their respiratory and pulmonary functions.Rats were divided into the following four groups; 25Gy left lung irradiation group, 17Gy bilateral lung irradiation group, 20Gy bilateral lung irradiation group and 25Gy bilateral lung irradiation group. The rats, having had their lungs irradiated in the Yamanashi University School of Medicine, were accepted in this research institute. We determined the body weight, body temperature and respiratory function every week and conducted pulmonary function determination, measurement of differential leukocyte counts of bronchoalveolar lavage fluid (BALF), arterial blood gas analysis, hematology, blood biochemistry and histopathological study every two weeks.Based on the results, changes in respiratory function, pulmonary function, hematological parameters, differen … More tial leukocyte counts of BALF and pulmonary fibrosis were confirmed following the irradiation of lungs in rats. To study the therapeutic effect of drugs on these irradiation-linked changes, a study conducted 6-8 weeks after irradiation including the 20Gy bilateral lung irradiation group, among the 25Gy left lung irradiation, 17Gy bilateral lung irradiation, 20Gy bilateral lung irradiation, and 25Gy bilateral lung irradiation groups, may be suitable.Next, we administrated 5 days of gefinitib(50mg/kg/body weight) and/or once cisplatin(10mg/kg/body weight) just before irradiation of 20Gy for bilateral rat lungs. The lung inflammation and fibrosis was increased by the administration of gefinitib and more by the addition of cisplatin in the findings of physiological pulmonary function and histopathology.In conclusion, a study conducted 6-8 weeks after irradiation including the 20Gy bilateral lung irradiation is a good model for a evaluation of a drug effect on rat radiation lung interstitial change, and we have to pay attention to the addition of gefinitib and/or cisplatin to lung radiotherapy. Less

本实验将大鼠随机分为4组：25 Gy左肺照射组、17 Gy双肺照射组、20 Gy双肺照射组和25 Gy双肺照射组，分别进行呼吸功能、肺功能测定、血液生化和组织学检查，研究照射对大鼠呼吸功能和肺功能的影响。在山梨大学医学院接受肺部照射的大鼠被该研究所接受。每周测定体重、体温和呼吸功能，每2周进行肺功能测定、支气管肺泡灌洗液（BALF）白细胞分类计数、动脉血气分析、血液学、血液生化学和组织病理学检查，根据结果观察呼吸功能、肺功能、血液学指标、肺组织病理学变化，并与对照组比较。 ...更多信息 大鼠肺照射后BALF中白细胞计数下降，肺纤维化。为了研究药物对这些放射相关变化的治疗作用，在25 Gy左肺照射组、17 Gy双肺照射组、20 Gy双肺照射组和25 Gy双肺照射组中，在照射后6-8周进行包括20 Gy双肺照射组的研究可能是合适的。在20 Gy照射大鼠双侧肺前给予吉非替布（50 mg/kg/体重）和/或顺铂（10 mg/kg/体重）1次。在生理肺功能和组织病理学的发现中，给予吉非替布增加了肺炎症和纤维化，并且加入顺铂增加了更多。总之，在照射后6-8周进行的研究，包括20 Gy的双侧肺照射，是评价药物对大鼠放射性肺间质变化的作用的良好模型，在肺放疗中应注意吉非替尼和/或顺铂的联合应用。少