Establishment of the radiosensitivity predicting system by comet assay using peripheral lymphocytes

外周淋巴细胞彗星试验放射敏感性预测系统的建立

• 批准号: 15591272
• 负责人: OYA Natsuo
• 金额: $ 2.11万
• 依托单位: Kumamoto University (2004)Kyoto University (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591272/
• 关键词: breast cancer; radiotherapy; radiation dermatitis; predictive assay; lymphocyte; comet assay; DNA repair; acute reaction; 放射線皮膚炎; 末梢リンパ球; DNA修復; 癌のオーダーメード治療

Twenty-nine patients with breast cancer who had developed Grade 2 acute radiation dermatitis following whole breast radiotherapy and 34 control patients who had not developed radiation dermatitis entered the present study. The patients' lymphocytes were irradiated at a dose of 12 Gy, and subjected to the single cell gel alkaline electrophoresis assay(comet assay) 0 to 180 minutes after irradiation. In most cases, standardized tail moment(STM) decreased with time, representing the repair procedure of radiation-induced DNA damage. The mean +/- SE of repair half-time(RHT), defined as the time required to reduce the STM to 50% of the STM at 0 min, in the dermatitis group and the control group was 31.0 +/- 4.8 min and 20.6 +/- 2.5 min(p=0.05), respectively. The results suggested that RHT, a parameter representing DNA repair velocity in ex vivo comet assay, may be a candidate for the radiation dermatitis predictor in patients receiving whole breast radiotherapy. Further evaluation of this assay in a prospective setting is indicated.

29例在全乳放疗后发生2级急性放射性皮炎的乳腺癌患者和34例未发生放射性皮炎的对照患者进入本研究。以12戈伊的剂量照射患者的淋巴细胞，并在照射后0至180分钟进行单细胞凝胶碱性电泳测定（彗星测定）。在大多数情况下，标准化尾矩（STM）随着时间的推移而减少，代表辐射诱导的DNA损伤的修复过程。在皮炎组和对照组中，修复半衰期（RHT）的平均+/- SE（定义为STM降至0 min时STM的50%所需的时间）分别为31.0 +/- 4.8 min和20.6 +/- 2.5 min（p=0.05）。结果表明，RHT，在体外彗星试验中代表DNA修复速度的参数，可能是接受全乳放疗患者的放射性皮炎预测的候选者。指出了在前瞻性环境中对该测定法的进一步评价。

项目成果

Prediction of radiotherapy-induced dermatitis by comet assay in ex vivo irradiated patients' lymphocytes

通过彗星试验预测离体受照射患者淋巴细胞的放疗引起的皮炎

• 发表时间: 2004
• 期刊: International Journal of Radiation Oncology Biology Physics 60
• 作者: N.Oya;H.Sai;S.Tachiiri;M.Mitsumori;Y.Nagata;M.Hiraoka
• 通讯作者: M.Hiraoka