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Biological modulation of steroid for bile output

类固醇对胆汁输出的生物调节

基本信息

批准号：
15591338
负责人：
YUASA Norihiro
金额：
$ 1.92万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

1.Peritoneal administration of steroid, 1.6 mg-dexamethazone, significantly increased basal biliary output in perfused rat liver.2.495±0.299 μL/min/g liver dexamethazone group2.152±0.083 μL/min/g liver control groupP=0.001622.Hydrogen sulfide is generated through cysteine metabolism with the catalytic enzyme such as cytathionine γ-lyase (CSE) in the liver. In the rat liver, the content of tissue hydrogen sulfide was around 80 μmol/g tissue, which was firstly measured by our gas-choromatography technique.3.Propagylglycine (PPG) administrated intraperitoneally, which was a potent inhibitor of CSE, significantly decreased tissue hydrogen sulfide level.4.The bile output and its constituents were evaluated in both in vivo and ex vivo perfusion system.(1)In the PPG-treated liver, basal biliary output significantly increased compared with that of control liver.(2)Supplementation of NaHS in the PPG-treated liver completely inhibited the PPG-effect on bile output in the prefused rat liver.(3)The elevation of basal bile output in the PPG-treated liver resulted from the increased excretion of biliary bicarbonate. And supplementation of NaHS also inhibited this PPG-effect.Hydrogen sulfide works as an endogenous gaseous modulator of the basal bile formation in the liver. And it modulates biliary bicarbonate excretion.

1.腹腔注射1.6 mg地塞米松可显著增加大鼠肝脏基础胆汁排出量，地塞米松组为2.495 ±0.299 μL/min/g肝脏对照组为2.152 ±0.083 μL/min/g肝脏对照组P = 0.001622。硫化氢是在肝脏中通过半胱氨酸代谢产生的。在大鼠肝脏中，组织硫化氢含量约为80 μmol/g组织，首次采用气相色谱法测定; 3.腹腔注射CSE抑制剂- (1)In PPG组基础胆汁排出量较对照组明显增加。（2）在PPG处理的大鼠肝脏中补充NaHS完全抑制PPG对大鼠肝脏胆汁分泌的影响。（3）PPG处理组基础胆汁排出量的增加是由于胆汁碳酸氢盐排泄量增加所致。硫化氢是肝脏基础胆汁生成的内源性气体调节剂。它调节胆汁碳酸氢盐排泄。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Carbon Monoxide Stimulates mrp2-Dependent Excretion of Bilirubin IX-α into the Perfused Rat Liever.

一氧化碳刺激 mrp2 依赖性胆红素 IX-α 排泄到灌注大鼠肝脏中。

DOI：
发表时间：
2003
期刊：
ANTIOXIDANTS & REDOX SIGNALING 5(4)
影响因子：
0
作者：
Shinji Norimizu;Atsushi Kudo;Mayumi Kajimura;Kazuo Ishikawa;Hisashi Taniai;Tokio Yamaguchi;Kimihito Fujii;Shigeki Arii;Yuji Nimura;Makoto Suematsu
通讯作者：
Makoto Suematsu

Norimizu S, et al.: "Carbon monoxide stimulates mrp2-dependent excretion of bilirubin-IXalpha into bile in the perfused rat liver"Antioxidants and Redox Signaling. 5(4). 449-456 (2003)

Norimizu S 等人：“一氧化碳刺激 mrp2 依赖性胆红素-IXα 排泄到灌注大鼠肝脏中的胆汁中”抗氧化剂和氧化还原信号传导。