Biological modulation of steroid for bile output

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1.Peritoneal administration of steroid, 1.6 mg-dexamethazone, significantly increased basal biliary output in perfused rat liver.2.495±0.299 μL/min/g liver dexamethazone group2.152±0.083 μL/min/g liver control groupP=0.001622.Hydrogen sulfide is generated through cysteine metabolism with the catalytic enzyme such as cytathionine γ-lyase (CSE) in the liver. In the rat liver, the content of tissue hydrogen sulfide was around 80 μmol/g tissue, which was firstly measured by our gas-choromatography technique.3.Propagylglycine (PPG) administrated intraperitoneally, which was a potent inhibitor of CSE, significantly decreased tissue hydrogen sulfide level.4.The bile output and its constituents were evaluated in both in vivo and ex vivo perfusion system.(1)In the PPG-treated liver, basal biliary output significantly increased compared with that of control liver.(2)Supplementation of NaHS in the PPG-treated liver completely inhibited the PPG-effect on bile output in the prefused rat liver.(3)The elevation of basal bile output in the PPG-treated liver resulted from the increased excretion of biliary bicarbonate. And supplementation of NaHS also inhibited this PPG-effect.Hydrogen sulfide works as an endogenous gaseous modulator of the basal bile formation in the liver. And it modulates biliary bicarbonate excretion.