Biological modulation of steroid for bile output

1.腹腔注射类固醇1.6 mg地塞米松可显著增加灌注大鼠肝脏基础胆输出量。地塞米松组（2.495±0.299 μL/min/g）肝组（2.152±0.083 μL/min/g）肝对照组（pp =0.001622）。硫化氢是肝脏中胱硫氨酸γ-裂解酶（CSE）等催化酶对半胱氨酸代谢产生的。在大鼠肝脏中，组织硫化氢含量在80 μmol/g左右，首次用气相色谱法测定。3 .腹腔注射丙基甘氨酸（PPG）可显著降低组织硫化氢水平，是一种有效的CSE抑制剂。在体内和离体灌注系统中评估胆汁输出量及其成分。(1)与对照组相比，ppg处理肝的基础胆输出量显著增加。(2)在ppg处理的肝脏中添加NaHS完全抑制ppg对预灌注大鼠肝脏胆汁输出的影响。(3) ppg治疗的肝脏基础胆汁输出量的升高是由于胆汁中碳酸氢盐的排泄增加。NaHS的补充也抑制了ppg效应。硫化氢是肝脏基础胆汁形成的内源性气体调节剂。它调节胆汁中碳酸氢盐的排泄。

1.Peritoneal administration of steroid, 1.6 mg-dexamethazone, significantly increased basal biliary output in perfused rat liver.2.495±0.299 μL/min/g liver dexamethazone group2.152±0.083 μL/min/g liver control groupP=0.001622.Hydrogen sulfide is generated through cysteine metabolism with the catalytic enzyme such as cytathionine γ-lyase (CSE) in the liver. In the rat liver, the content of tissue hydrogen sulfide was around 80 μmol/g tissue, which was firstly measured by our gas-choromatography technique.3.Propagylglycine (PPG) administrated intraperitoneally, which was a potent inhibitor of CSE, significantly decreased tissue hydrogen sulfide level.4.The bile output and its constituents were evaluated in both in vivo and ex vivo perfusion system.(1)In the PPG-treated liver, basal biliary output significantly increased compared with that of control liver.(2)Supplementation of NaHS in the PPG-treated liver completely inhibited the PPG-effect on bile output in the prefused rat liver.(3)The elevation of basal bile output in the PPG-treated liver resulted from the increased excretion of biliary bicarbonate. And supplementation of NaHS also inhibited this PPG-effect.Hydrogen sulfide works as an endogenous gaseous modulator of the basal bile formation in the liver. And it modulates biliary bicarbonate excretion.