Irradiation for Ex vivo graft to prevent the small-bowel rejection

离体移植物辐射预防小肠排斥反应

• 批准号: 15591366
• 负责人: SUGITO Kiminobu
• 金额: $ 1.86万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591366/
• 关键词: small bowel transplantation; 放射線照射

MAdCAM-1 expression on HEVs in PPs was down-regulated during rejection. In contrast its expression on endothelial cells of vessels in the lumina propria was up-regulated during rejection. On day 5 the number of Fas- and FasL-postive enterocytes were increased significantly in the allografts compared with the nontransplanted controls. Similarly, in the PPs, Fas- and FasL-positive mononuclear cells also increased significantly on day 5 in the allografts. Alteration in MAdCAM-1 expression may be associated with the development of SB graft rejection. Activation of fes/FasL system occurred during SB graft rejection suggest that it may be associated with the development of SB graft rejection.We also studied about the effect of ex vivo graft irradiation and FTY720. Graft survival was prolonged in FTY720 model and ex vivo graft irradiation +FTY720 model. In FTY720 model and ex vivo graft irradiation +FTY720 model, the number of the expression of MAdCAM-1 expression on HEVs in PPs and, Fas/FasL-positive mononuclear cells in the PPs were decreased.These findings suggest that ex vivo graft irradiation +FTY720 is important for the control of SB graft rejection.

排斥反应时，PPS中HEV上MAdCAM-1的表达下调。相反，在排斥反应期间，其在固有腔血管内皮细胞上的表达上调。移植后第5天，移植物中Fas和FasL阳性的肠上皮细胞数明显多于未移植对照组。同样，在移植后第5天，PPS中Fas和FasL阳性的单个核细胞也显著增加。MAdCAM-1表达的改变可能与SB移植物排斥反应的发生有关。Sb移植物排斥反应过程中FES/FasL系统的激活可能与Sb移植物排斥反应的发生有关。我们还研究了体外移植物照射和FTY720的影响。FTY720模型和体外照射+FTY720模型移植物存活时间延长。在FTY720模型和体外照射+FTY720模型中，PPS中HEV上MAdCAM-1的表达和PPS中Fas/FasL阳性的单个核细胞数均减少，提示体外照射+FTY720对控制SB移植物排斥反应具有重要意义。

项目成果

Semiquantative Analysis of Expression of Mucosal Addressin Cell Adhesion Molecule-1 During Small Bowel Rejection in Rats

大鼠小肠排斥过程中粘膜寻址细胞粘附分子-1表达的半定量分析

• 发表时间: 2004
• 期刊: Transplantation Proceeding 36
• 作者: S.Fujisaki;R.Tomita;Y.J.Park;K.Kimizuka;K.Sugito;M.Inoue;M.Fukuzawa
• 通讯作者: M.Fukuzawa

Apotosis of Cript Cells and Lymphocytes in Gut-Associated Lymphoid Tissues During Small Intestinal Graft Rejection in Rats

大鼠小肠移植排斥过程中肠道相关淋巴组织中隐细胞和淋巴细胞的凋亡

• 发表时间: 2004
• 期刊: Transplantation Proceedings 36
• 作者: Y.J.Park

Effect of a novel immunosuppressant FTY720 in mice without secondary lymphoid organs

新型免疫抑制剂 FTY720 对无次级淋巴器官小鼠的影响

• 发表时间: 2003
• 期刊: Japanese Journal of Transplantation 38
• 作者: Y.J.Park;S.Fujisaki;K.Kimizuka;K.Sugito;R.Tomita;M.Fukuzawa;K.Matsumoto;藤崎 滋;杉藤 公信;杉藤 公信;朴 英智;S.Fujisaki;K.Sugito
• 通讯作者: K.Sugito

小腸移植拒絶反応における腸管粘膜防御機構の破碇-特にpeyer板の変化に関して

小肠移植排斥反应中肠粘膜防御机制的破坏 - 特别是派尔氏板的变化

• 发表时间: 2002
• 期刊: The Japanese Society of Digestion and Absorption 25
• 作者: Y.J.Park;S.Fujisaki;K.Kimizuka;K.Sugito;R.Tomita;M.Fukuzawa;K.Matsumoto;藤崎 滋;杉藤 公信;杉藤 公信;朴 英智;S.Fujisaki;K.Sugito;K.Sugito;藤崎 滋
• 通讯作者: 藤崎 滋

Apoptosis of Crypt Cells and Lymphocytes in Gut-Associated Lymphoid Tissues During Small Intestinal Graft Rejection in Rats

大鼠小肠移植排斥反应中肠道相关淋巴组织隐窝细胞和淋巴细胞的凋亡

• 发表时间: 2004
• 期刊: Transplantation Proceeding 36
• 作者: Y.J.Park;S.Fujisaki;K.Kimizuka;K.Sugito;R.Tomita;M.Fukuzawa;K.Matsumoto
• 通讯作者: K.Matsumoto