Difference in the anti-proliferative effect by cholecalciferol between sublines of a pancreatic cancer cell lines and various genes expressions examined by cDNA array

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To examine the difference in the anti-proliferative effect by cholecalciferol between pancreatic cancer cells by cholecalciferol, four sublines of a pancreatic cancer cell line SUIT-2,S2-007,S2-013,S2-020, and S2-028 were incubated at varied concentrations of cholecalciferol and the cellular proliferation were assessed by MIT assay. Messenger RNA levels of vitamin D receptor (VDR) were also measured by quantitative RT-PCT. As a result cholecalciferol inhibited the cellular proliferation depending on the concentrations (10^<-6> M,10^<-7> M,10^<-8> M), most strongly in S2-028, moderately in S2-007 and S2-013, and weekly in S2-002. Quantitative reverse transcriptase polymerase chain reaction showed higher constitutional gene expression of VDR mRNA in S-028 and greater induction by cholecalciferol than those in the other sublimes. To investigate the gene expressions, related to cellular proliferation and metastasis in these sublines, cDNA array analysis was performed in genes as follows : ACTB,AMFR,FGF2,CTSB,PECAM1,CD34,CDH1,EGFR,ETS1,FLT1,FLT4,FOS,KDR,MMP1,MMP2,MMP3,MMP7,MMP9,MET,MMP14,MMP16,MYC,RELA,NME1,NME2,SERPINE1,SERPINB2,TFRC,TGFA,TGFB1,TGFB2,TGFB3,TGFBR3,TIE,TEK,PLAU,PLAUR,VEGF,VEGFC,ITGA2,ITGA3,ITGA4,ITGA5,ITGA6,CTNNA1,ITGAV,THBS1,HTGB1,ITGB3,ITGB5,CTNNB1,AHCY,CBS,DNMT1,DNMT3A,HDAC1,HDAC2,HDAC3,HDAC4,HDAC5,HDAC6,MAT1A,MAT2A,MAT2B,MBD2,MBD3,MBD4,MECP2,MTHFR,MTR,CTSL,ANGPT2. As a result mRNA expression levels were higher than those of the other sublines in nine genes such as EGFR,MMP1,MMP3,MMP7,TGFB2,TGFBR3,PLAU,ITGA2, and ANGPT2. S2-028 showed higher gene expression than those in the other sublines.