Study of expression mechanism of laminin-5 γ2 chain in colon cancer cell lines

MMP-7和laminin-5 γ2链在癌细胞细胞质中的表达(1)在L-10和HT-29细胞中，MMP-7和laminin-5 γ2链在癌细胞细胞质中均有表达，而在SW620、col320d、LS174T细胞中，这些蛋白不表达。队列迁移模型(2)正如Nabeshima等人提出的，在L-10细胞中确实观察到了队列迁移模型，而在其他细胞系中没有观察到队列迁移模型。L-10细胞在组织培养玻片上形成相互连接、堆积的细胞岛，部分癌细胞从细胞岛向外迁移到细胞岛间空间，形成单细胞厚的相干细胞片。MMP-7和laminin-5 γ - 2链均在迁移癌细胞细胞质中表达shgf /SF刺激试验(3)重组肝细胞生长因子/分散因子（HGF/SF）刺激后，迁移的L-10细胞数量较未刺激时显著增加。(4)当L-10细胞在抗h MMP-7（+）培养基中培养时，观察到队列迁移的方式与在普通培养基中培养时相同。MMP-7和laminin-5 γ - 2链的表达模式也相似。

MMP-7 and laminin-5 γ2 chain expression in cancer cell lines(1) In L-10 and HT-29 cells, both MMP-7 and laminin-5 γ2 chain were expressed in the cytoplasm of cancer cells, whereas in SW620, Colo320D, LS174T cells these proteins were not expressed.Cohort migration model(2) As Nabeshima et al.suggested, cohort migration model was actually observed in L-10 cells, however, it was not observed in the other cell lines examined. L-10 cells formed interlinked, piled-up cell islands on the tissue culture glass slides, and some cancer cells migrated outward from the cell islands into inter-island spaces as coherent cell sheets with one-cell thick. Both MMP-7 and laminin-5 γ2 chain were expressed in the cytoplasm of migrated cancer cellsHGF/SF stimulation test(3) When stimulated with recombinant hepatocyte growth factor/scattered factor (HGF/SF), the number of migrated L-10 cells were significantly increased as compared with that without stimulation.Suppression test(4) When L-10 cells were cultured in the anti-h MMP-7 (+) medium, cohort migration was observed as in the same fashion as when cultured in the ordinary medium. The expression patterns of MMP-7 and laminin-5 γ2 chain were also similar.