Study of expression mechanism of laminin-5 γ2 chain in colon cancer cell lines

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MMP-7 and laminin-5 γ2 chain expression in cancer cell lines(1) In L-10 and HT-29 cells, both MMP-7 and laminin-5 γ2 chain were expressed in the cytoplasm of cancer cells, whereas in SW620, Colo320D, LS174T cells these proteins were not expressed.Cohort migration model(2) As Nabeshima et al.suggested, cohort migration model was actually observed in L-10 cells, however, it was not observed in the other cell lines examined. L-10 cells formed interlinked, piled-up cell islands on the tissue culture glass slides, and some cancer cells migrated outward from the cell islands into inter-island spaces as coherent cell sheets with one-cell thick. Both MMP-7 and laminin-5 γ2 chain were expressed in the cytoplasm of migrated cancer cellsHGF/SF stimulation test(3) When stimulated with recombinant hepatocyte growth factor/scattered factor (HGF/SF), the number of migrated L-10 cells were significantly increased as compared with that without stimulation.Suppression test(4) When L-10 cells were cultured in the anti-h MMP-7 (+) medium, cohort migration was observed as in the same fashion as when cultured in the ordinary medium. The expression patterns of MMP-7 and laminin-5 γ2 chain were also similar.