Instability and remodeling of cerebral aneurysmal walls

脑动脉瘤壁的不稳定和重塑

• 批准号: 15591528
• 负责人: NAGAHIRO Shinji
• 金额: $ 2.11万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591528/
• 关键词: aneurysm; smooth muscle cell; macrophage; BTEB2; rat model; estrogen; ovary

1)Unruptured and ruptured aneurysms were obtained at surgery or autopsy : control cerebral arteries at autopsy. The specimens were stained immunohistochemically with antibodies to α-smooth muscle actin, macrophages, and basic transcription regulatory element binding protein 2 (BTEB2). BTEB2-positive macrophages and smooth muscle cells were found in the luminal layer of large and giant unruptured aneurysms. This suggests that BTEB2 expression in the aneurysmal wall may play a role in the growth of aneurysms and may protect against their rupture.2)We used vascular corrosion casts in order to detect early ultrastructural changes that occur at cerebral arterial bifurcations in response to hemodynamic stress and lead to cerebral aneurysms formation in rats subjected to aneurysm induction by ligation of the right common carotid artery and bilateral posterior renal arteries. Of the 20 operated rats, 11 (55%) developed aneurysmal changes ; 5 of these manifested changes of the endothelial cell imprints only (Stage I). In the other 6 rats, morphological changes of endothelial cells were associated with different grades of aneurysmal dilatation : 2 rats developed saccular dilatation of the arterial bifurcation (stage III) and 4 manifested intimal pad elevation (stage II).3)New experimental aneurysmal model was developed in female rats subjected to estrogen deficiency by adding oophorectomy. Frequency of aneurysm formation in oophorectomized rats was 3 times higher than that in rats without oophorectomy. Frequency of aneurysm formation in rats treated with estrogen replacement reduced to the control level. The findings suggest that estrogen play an important role in the pathogenesis of cerebral aneurysms.

1)手术或尸检获得未破裂和破裂的动脉瘤：尸检时控制脑动脉。对标本进行α-平滑肌肌动蛋白、巨噬细胞和碱性转录调节元件结合蛋白2(BTEB2)免疫组织化学染色。未破裂的大、巨型动脉瘤的管腔内可见BTEB2阳性的巨噬细胞和平滑肌细胞。这表明BTEB2在动脉瘤壁上的表达可能在动脉瘤的生长过程中起作用，并可能对动脉瘤的破裂起到保护作用。2)我们采用血管铸型技术，通过结扎右侧颈总动脉和双侧肾后动脉的方法，观察脑动脉分叉处对血流动力学应力的早期超微结构变化，并导致脑动脉瘤的形成。在20只手术大鼠中，11只(55%)发生了动脉瘤样变，其中5只只表现为内皮细胞印迹的变化(I期)。另6只大鼠血管内皮细胞形态改变与不同程度的动脉瘤扩张有关：2只大鼠出现动脉分叉部囊状扩张(III期)，4只大鼠出现内膜垫抬高(II期)。3)雌激素缺乏雌性大鼠加卵巢切除建立新的实验性动脉瘤模型。去卵巢大鼠的动脉瘤发生率是未去卵巢大鼠的3倍。在接受雌激素替代治疗的大鼠中，动脉瘤形成的频率降低到对照水平。提示雌激素在脑动脉瘤的发病机制中起重要作用。

项目成果

Vascular corrosion cast mirrors early aneurysmal morphological changes

血管腐蚀铸件反映了早期动脉瘤的形态变化

• 发表时间: 2004
• 期刊: Journal of Neurosurgery 102(in press)
• 作者: Mohammad A.Jamous;Shinji Nagahiro;Keiko T.Kitazato;Koichi Satoh;Junichiro Satomi
• 通讯作者: Junichiro Satomi

Ruptured aneurysm arising from a basilar artery fenestration and associated with a persistent primitive hypoglossal artery

由基底动脉开窗引起并与持续的原始舌下动脉相关的破裂动脉瘤

• 发表时间: 2004
• 期刊: Journal of Neurosurgery 101
• 作者: Fumoto N;Nakatsuka H;Ohta S;Kumon Y;Ohnishi T;Hamada J. et al.;Miyuki Kanematsu
• 通讯作者: Miyuki Kanematsu

Incidence and risk factors for the growth of unruptured cerebral aneurysms : observation using serial computerized tomography angioaraphy

未破裂脑动脉瘤生长的发病率和危险因素：使用连续计算机断层扫描血管治疗法观察

• 发表时间: 2004
• 期刊: Journal of Neurosurgery 101
• 作者: 藤井正美;鈴木倫保他;Shunji Matsubara
• 通讯作者: Shunji Matsubara

Ischemic basilar artery dissecting aneurysm treated by stenting only

仅支架置入术治疗缺血性基底动脉夹层动脉瘤

• 发表时间: 2004
• 期刊: Neurol Med Chir (Tokyo) 44
• 作者: Jamous MA;Satoh K;Matsubara S;Satomi J;Nakajima N;Uno M;Nagahiro S.
• 通讯作者: Nagahiro S.

Primitive vertebrovasilar system associated with a Ruptured aneurysm

与破裂动脉瘤相关的原始椎血管系统

• 发表时间: 2004
• 期刊: AJNR 25
• 作者: K.Yagi;K.Satoh;J.Satomi;S.Nagahiro
• 通讯作者: S.Nagahiro