Organization of Electrolodes for the Electrochemotherapy for Deep-seated Malignant Musculoskeletal Tumor

A.我们对荷瘤小鼠和兔进行电化学治疗后的肿瘤坏死面积进行了组织学评估。电化学治疗是用两个电极进行的，放置电极以捕获肿瘤负荷。对肿瘤坏死面积进行三维评估。通过在肿瘤周围的不同部位应用多个电极以引起对肿瘤负荷的多次治疗，获得了有效的治疗。声致孔作为一种有效的药物传递系统，被应用于肿瘤治疗。声致孔与电穿孔一样，是一种对细胞膜进行瞬时穿刺的方法。声致穿孔被认为比电穿孔更安全，因为其副作用更少，例如引起烧伤。甲氨蝶呤常用于治疗全身性关节炎，如类风湿性关节炎。甲氨蝶呤关节内给药曾被用于研究，但 ...更多信息 认为没有获得有效的结果，因为甲氨蝶呤本身不容易通过细胞膜。采用甲氨蝶呤关节腔内注射，体外超声刺激声孔，治疗兔膝关节炎，经病理学检查证实，治疗有效。联合应用甲氨蝶呤和超声影像增强器可增强疗效。该图像增强器由微泡组成，微泡通过超声刺激而破裂，从而导致局部水流刺穿细胞膜。这种新的药物传递系统可以应用于各种病理条件，如肿瘤治疗或全身性关节炎。C.作为一种新的治疗肌肉骨骼肿瘤，我们应用化疗联合声孔治疗大腿恶性肿瘤在我们大学的机构审查委员会。临床试验取得了良好的效果，无任何副作用。少

A.We performed histologic evaluation of the area of tumor necrosis after electrochemotherapy to tumor-bearing mouse and rabbit Electrochemotherapy was done with two electrolodes that was placed to catch the tumor burden. The area of tumor necrosis was evaluated three dimensionally. By applying many electrolodes that were placed various sites around the tumor in order to cause multiple treatments to the tumor burden, effective treatment was observed.B. As an effective drug delivery system, we apply sonoporation to tumor treatment Sonoporation is same kind of method for transient puncture to cell membrane as electroporation. Sonoporation was thought to be safer than electroporation in the point of fewer side effects such as causing bum. We apply sonoporation as intra-articular treatment of rabbit knee joint Methotrexate is often used for the treatment of generalized arthritis, such as rheumatoid arthritis. Intra-articular administration of methotrexate was used to be studied, but it was … More believed that effective result was not obtained, because methotrexate itself was not easily passed through the cell membrane. Intra-articuular injection of methotrexate followed by extracorporeal ultrasonographic stimulation, sonoporation, was applied to the experimentanry-established arthritis in the knee joint of rabbit Histopathologic evaluation revealed effective treatment for arthritis. Furthermore, combined solution, methotrexate and ultrasonic image intensifier, enhanced the effect. This image intensifier is consisting of microbubble that is broken by ultrasonic stimulation, which causes local water stream punctuating the cell membrane. This new drug delivery system can apply to various pathological condition such as tumor treatment or systemic arthritis.C.As a new treatment for musculoskeletal tumor, we applied chemotherapy combined with sonoporation to thigh malignant tumor under institutional review board of our university. This clinical trial achieved good result without any side effect. Less