Gale therapy for osteoarthritis

骨关节炎的大风疗法

• 批准号: 15591585
• 负责人: WATANABE Shohei
• 金额: $ 2.3万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591585/
• 关键词: Gene therapy; Osteoarthritis; Adenovirus; SRT; ort mice; IL-4; FGF2; IL4; プラスミド

1.To determine the therapeutic effect of IL-4 mediated adenovirus vector on the murine osteoarthritis model, AdCIL4 was administrated to the male 16-week-old SRT/ort mice intravenously. It was observed that knee joints form the mice to which injected with AdCIL4 showed significantly inhibited to affect with osteoarthritic changes compared with those of control mice. Although there was the problem to induce the inflammation by AdCIL4, it seemed that local administration with vectors could be a therapeutic method for osteochondral lesion.2.To examine the effect of IL-4 on matrix synthesis in chondrocytes under excessive mechanical stress in vitro. Mechanical stress for 16 hours significantly decreased levels of mRNA for both AGG and CII (p<0.01), but rat IL-4 at doses of 1 or 10 ng/ml recovered these levels (p<0.05).These findings suggested that IL-4 might prevent articular cartilage from damage caused by mechanical stress and therefore could be a useful therapeutic agent for treatment of arthritic disorders.

1.为探讨IL-4腺病毒载体对小鼠骨关节炎模型的治疗作用，将AdCIL-4静脉注射给16周龄雄性SRT/ort小鼠。观察到，与对照小鼠相比，注射AdCIL 4的小鼠的膝关节显示出对骨关节炎变化的显著抑制作用。虽然AdCIL-4在诱导炎症反应方面存在一定的问题，但局部给药有望成为治疗骨软骨损伤的一种方法。2.研究IL-4对体外培养的软骨细胞在过度机械应力下基质合成的影响。机械应力作用16小时后，AGG和CII的mRNA水平均显著降低（p<0.01），而IL-4在1和10 ng/ml剂量下可使AGG和CII的mRNA水平恢复（p<0.05）。

项目成果

Interleukin-4 protects matrix synthesis in chondrocytes under excessive mechanical stress in vitro.

Interleukin-4 在体外过度机械应力下保护软骨细胞的基质合成。

• 发表时间: 2004
• 期刊: Modern Rheumatology 14
• 作者: A.Shimizu;S.Watanabe;S.Iimoto;H.Yamamoto
• 通讯作者: H.Yamamoto

Recombinant adeno-associated virus preferentially transduces human, compared to mouse, synovium : implications for arthritis therapy.

与小鼠相比，重组腺相关病毒优先转导人类滑膜：对关节炎治疗的影响。

• 发表时间: 2004
• 期刊: Modern Rheumatology 14
• 作者: Shigeki Katakura;Kristi Jennings;Shohei Watanabe;et al.
• 通讯作者: et al.

Interleukin-4 protects matrix synthesis in chondrocytes under excessive mechanical stress in vitro

Interleukin-4 在体外过度机械应力下保护软骨细胞的基质合成

• 发表时间: 2004
• 期刊: Modern Rheumatology 14, (4)
• 作者: Akira Shimizu;Shohei Watanabe;Seiji Iimoto;et al.
• 通讯作者: et al.

Recombinant adeno-associated virus preferentially transduces human, compared to mouse, synovium : implications for arthritis ther

与小鼠滑膜相比，重组腺相关病毒优先转导人类滑膜：对关节炎的影响

• 发表时间: 2004
• 期刊: Modern Rheumatology 14, (1)
• 作者: Shigeki Katakura;Kristi Jennings;Shohei Watanabe;et al.
• 通讯作者: et al.