INVESTIGATION INTO THE ROLE OF POTASSIUM CHANNELS IN THE LOW-OXYGEN SENSING MECHANISM IN OF AIRWAY CHEMORECEPTORS

钾离子通道在气道化学感受器低氧传感机制中的作用研究

• 批准号: 15591663
• 负责人: KOBAYASHI Noriyuki
• 金额: $ 2.11万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591663/
• 关键词: oxygen sensing mechanism; pulmonary epitherial cells; carotid body; potassium channels; 頸動脈小体

We spent a lot of time to make an electrophysiological system of patch clump. But finally we could investigate and analyze TASK-1 mRNA, one of the leak potassium channels, in neuroepitherial bodies of the lung. In histology, we could also detect TASK-1 channel in the same tissue. Ikn were enhanced by halothane and inhibited by anandamide. These observations demonstrates that TASK-1 channel play an important role in airway chemoreception. It is well known that oxygen sensing mechanisms in airway and arterial chemoreceptors are thought to be the same. We have already made clear only TASK-1 channel had a crucial role, but not Kv or BK channels in the oxygen sensing mechanisms of the carotid body as an arterial chemoreceptor, in Karolinska Institute two years ago. We had made a comparison our new data between old one in Karolinska Institute. We announced a part of our results in 13^<th> World Congress of Anaesthesiologists, Paris April, 2004. We will contribute an article of this research.

我们花了很多时间制作了一个贴片块的电生理系统。但最终我们可以研究和分析肺神经上皮体中的钾离子通道之一--Task-lmRNA。在组织学上，我们也可以在同一组织中检测到ASK-1通道。氟烷可增强IKN，花生四烯酸则可抑制IKN。这些观察结果表明，在气道化学感受中，ASK-1通道起着重要作用。众所周知，气道和动脉化学感受器中的氧感测机制被认为是相同的。两年前，我们已经在Karolinska研究所明确指出，在颈动脉体作为动脉化学感受器的氧感受机制中，只有ASK-1通道起关键作用，而不是Kv或BK通道。我们将新数据与卡罗林斯卡医学院的旧数据进行了比较。我们在<th>2004年4月于巴黎举行的第13届世界麻醉医师大会上公布了部分结果。我们将贡献一篇关于这项研究的文章。