Analysis and identifying adhesion molecules on endometrial epithelial cells that induce and activate embryo

分析和鉴定子宫内膜上皮细胞上诱导和激活胚胎的粘附分子

• 批准号: 15591743
• 负责人: KOSAKA Kenzo
• 金额: $ 2.18万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591743/
• 关键词: endometrial epithelial cells; embryo; adhesion molecule; peripheral blood mononuclear cells; DNA array; Eph-ephrin; 抹消血単核球

This study demonstrated that ephrin A1 is expressed on human endometrial epithelial cells. On the other hand, mRNA expression of Eph A1 was detected in human embryos in the blastocyst stage, suggesting a physiological role of the Eph-ephrin system in embryo implantation. These findings indicate a possible interaction between human blastocysts and endometrial epithelial cells via the Eph-ephrin system. Because intracytoplasmic signals are induced by Eph receptors after ephrin stimulation, this system may be involved in the activation process of the human embryo during the implantation period.Also, this study demonstrated that ALCAM is expressed on human endometrial epithelial cells and blastocysts. The developing stage-specific expression on the embryo suggests that the ALCAM-ALCAM cell adhesion system is involved in an initial interaction of the embryo with maternal endometrium.Further investigation of these expression and functions of these molecules will provide new insights into the mechanisms of the initial embryo-maternal interaction.

本研究证实ephrin A1在人子宫内膜上皮细胞上表达。另一方面，Eph A1的mRNA表达在人胚胎的囊胚期，表明Eph-ephrin系统在胚胎植入的生理作用。这些发现表明，人胚泡和子宫内膜上皮细胞之间可能通过Eph-ephrin系统的相互作用。由于Eph受体在Ephrin刺激后可诱导胞浆内信号，该系统可能参与了人胚胎着床期的激活过程。此外，本研究证实ALCAM在人子宫内膜上皮细胞和囊胚中均有表达。ALCAM-ALCAM细胞粘附系统在胚胎发育阶段特异性表达，提示ALCAM-ALCAM细胞粘附系统参与了胚胎与母体子宫内膜的初始相互作用，进一步研究这些分子的表达和功能将为胚胎-母体初始相互作用机制提供新的认识。

项目成果

Egawa, M.: "Ephrin B1 is expressed on human luteinizing granulosa cells in corpora lutea of the early luteal phase : the possible involvement of the B class Eph-ephrin system during corpus luteum formation"J.Clin.Endocrinol.Metab.. 88(9). 4384-4392 (2003)

Ekawa, M.：“Ephrin B1 在黄体早期黄体中的人黄体化颗粒细胞上表达：B 类 Eph-ephrin 系统在黄体形成过程中可能参与”J.Clin.Endocrinol.Metab.. 88

Higuchi, T.: "Cyclic AMP enhances the expression of an extravillous trophoblast marker, melanoma cell adhesion molecule, in choriocarcinoma cell JEG3 and human chorionic villous explant cultures"Mol.Hum.Reprod.. 9(6). 359-366 (2003)

Higuchi, T.：“环状 AMP 增强绒毛膜癌细胞 JEG3 和人绒毛膜绒毛外植体培养物中绒毛外滋养层标记物、黑色素瘤细胞粘附分子的表达”Mol.Hum.Reprod.. 9(6)。

Kosaka K: "Human peripheral blood mononuclear cells enhance cell-cell interaction between human endometrial epithelial cells and BeWo-cell spheroids"Hum Reprod. 18(1). 19-25 (2003)

Kosaka K：“人外周血单核细胞增强人子宫内膜上皮细胞和 BeWo 细胞球体之间的细胞间相互作用”Hum Reprod。

Fujiwara, H: "Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD 166)"J.Clin.Endocrinol.Metab.. 88(7). 3437-3443 (2003)

Fujiwara, H：“人类囊胚和子宫内膜上皮细胞表达活化的白细胞细胞粘附分子 (ALCAM/CD 166)”J.Clin.Endocrinol.Metab.. 88(7)。

Human peripheral blood mononuclear cells enhance cell-cell interaction between human endometrial epithelial cells and BeWo-cell spheroids

人外周血单核细胞增强人子宫内膜上皮细胞与BeWo细胞球体之间的细胞间相互作用

• 发表时间: 2003
• 期刊: Hum Reprod. 18(1)
• 作者: Kosaka;K.
• 通讯作者: K.