Examination of the gene therapy for OME using Decoy oligo.

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TNF-a induced or upregulated IL-8 production in adenoidal fibroblasts. IL-8 level in epipharyngeal effusion and the compliance of tympanic membrane was showed clinically to be correlated in OME, suggesting that I L-8 production from adenoidal fibroblasts is one of the important causative factors of OME. Additionally, VCAM-1 was induced by the stimulation of TNF-a and/or IL-4 in nasal fibroblasts.Translocation of NF-κB is the essential step both in IL-8 production in adenoidal fibroblasts and VCAM-1 production from nasal fibroblasts.Based on these data, NF-κ B is the key factor and the regulation of this step seems to be a new strategy to treat OME and C S.In this project, NF-κB Decoy oligo was expected to suppress the activation of NF-IC B translocation. We applied the liposome method and sendai virus vector to transfect NF-κ B Decoy oligo to cultured fibroblast. However, the transfection was not successful and we could not go to the step of the application of this strategy to the OME mouse model.