A study on the expression of villin in taste organs : establishment of the villin-immunoreactive cells as taste receptors.

我调查了从哺乳动物到鱼类的15种脊椎动物的味觉器官的绒毛，这是一个桥接分子的细胞骨架蛋白，肌动蛋白的荧光免疫组织化学的手段。绒毛蛋白免疫反应性细胞出现在本研究中使用的所有物种的器官。此外，在青蛙味觉器官中，绒毛蛋白免疫阳性细胞被鉴定为受体细胞（Yoshie et al.，2003).免疫染色的哺乳动物味蕾细胞绒毛蛋白同时对IP 3R-3、PLCβ2和gustducin呈免疫反应性。电镜免疫细胞化学结果显示，大鼠胃粘膜中的I型和II型细胞仅被免疫染色，推测这些免疫染色的细胞具有味觉感受器的功能。

I investigated taste organs of 15 species of vertebrate animals from mammals to fish by mean of fluorescence immunohistochemistry for villin, which is a bridging molecule of the cytoskeletal protein, actin. Cells immunoreactive for villin appeared in the organs of all the species used in this study. In the frog taste organ, furthermore, villin-immunopositive cells were identified as receptor cells (Yoshie et al., 2003).Mammalian taste-bud cells immunostained for villin were concomitantly immunoreactive for IP3R-3, PLCβ2, and gustducin. The electron-microscopic immunocytochemistry for villin in rats exhibited that type I and type II cells were exclusively immunostained.Considering these results, the villin-immunostained cells were postulated to function as taste receptors.