A Fundamentel Study of Mineral Trioxide Aggregate(MTA)-Biological Effects to Dental Pulp and Periodontal Tissues and Behaviours of Related Cells-

三氧化二矿物质聚集体（MTA）的基础研究-对牙髓和牙周组织的生物效应及相关细胞的行为-

• 批准号: 15592031
• 负责人: OGISO Bunnai
• 金额: $ 1.86万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592031/
• 关键词: MTA; Calcified Tissue Formation; Rat Experimental Model; Rat Bone Marrow Stromal Cell; Immuno-Histlogical Staining; Bone Related Proteins; ラット属髄細胞; 骨連タンパク; 硬組織形成能; ラット頭蓋骨モデル; 病理組織学的検討; 免疫組織化学的検討

Aim of these consecutive studies was to investigate biological effects of Mineral Trioxide Aggregate(MTA) to calcified tissue formation from in vivo and in vitro assessments.Experiment 1 : Immuno-histological assessment of new calcified tissue formation promoted by MTA on Rat experimental modelFirst of all, the in vivo study with a rat experimental model has been performed in orda to evaluate effect of MTA for new bone formation. In this study, the ability of calcified tissue formation after the implantation of MTA in a rat parietal bone. IRM(Intermediate Restorative Material) was used as a control. MTA was encapsulated by the fibrous csonnective tissue in which PCNA and Cbfa-1 positive cells with some new calcified tissue formation was immuno-histologically observed. Otherwise, IRM was also encapsulated by the fibrous connective tissue, but any calcified tissue formation and Cbfa-1 positive cells were not founded. Those phenomena suggested that MTA might induce the difierentiation of … More undifferentiated pre-osteoblastic cells to osteogenic cells and on the other hand, IRM might be a low biocompatible material.Experiment 2 : Behaviour of osteoblast-like cells on MTA associated with expression of type I collagen and bone related protein mRNAsSecondary, the in vitro study was performed to investigate a behaviour of rat bone marrow cells(RBM), osteoblast-like cells, on MTA compared with IRM. RBM obtained from rat femur and cultured were used in this study. The cultured RBM were seeded on each material, and they were evaluated morphologically using scanning(SEM) and transmission(TEM) electron microscopies. Furthermore, the calcium released hydrorated material, the cell proliferation ratio and alkaline phosphatase(ALP) activity were analyzed, and the expression of type I collagen and bone related protein mRNAs were evaluated.SEM showed that RBM attached to MTA and had a flattened appearance without nuclear protrusions and microspikes. TEM showed that the cells attached in the same manner as the control group, but gaps lager than 2 μm were frequently observed. The calcium released from hydrated MTA was about 130 ppm after 3days of immersion in saline. The ALP activity was similar to the control group. Cell proliferation and expression of type I collagen mRNA was significantry lower, while the expression of osteopontin mRNA was significantly higher than the control group at the 3^<rd> day of culture. In IRM groups, a few rounded cells were observed on the material but no living cells were seen. Less

这些连续研究的目的是从体内和体外评估中研究矿物三氧化物聚集体（MTA）对钙化组织形成的生物学效应。实验1：MTA促进大鼠实验模型新钙化组织形成的免疫组织学评估首先，用大鼠实验模型进行了体内研究，以评估MTA对新骨形成的作用。在这项研究中，MTA植入大鼠顶骨后，钙化组织形成的能力。中间修复材料（Intermediate Restorative Material）用作对照。免疫组化显示MTA被纤维连接包裹，纤维连接内有PCNA和Cbfa-1阳性细胞，并有新的钙化组织形成。除此之外，纤维结缔组织也包裹着钙化组织，但未发现任何钙化组织形成和Cbfa-1阳性细胞。这些现象提示MTA可能诱导了细胞分化， ...更多信息 实验2：成骨样细胞在MTA上的行为与I型胶原和骨相关蛋白mRNA的表达相关其次，进行体外研究以研究大鼠骨髓细胞（RBM），成骨样细胞与MTA相比在MTA上的行为。本研究使用从大鼠股骨获得并培养的RBM。将培养的RBM接种在每种材料上，并使用扫描（SEM）和透射（TEM）电子显微镜对其进行形态学评价。扫描电镜显示RBM与MTA结合，细胞表面平整，无核突起和微刺，细胞增殖率、碱性磷酸酶（ALP）活性、I型胶原和骨相关蛋白mRNA表达均较正常对照组高。透射电镜显示细胞贴壁方式与对照组相同，但常可见大于2 μm的间隙。在盐水中浸泡3d后，从水合MTA中释放的钙约为130 ppm。ALP活性与对照组相似。与对照组相比，培养第3天，细胞增殖和I型胶原mRNA表达显著降低，而骨桥蛋白mRNA表达显著升高<rd>。在植入组中，在材料上观察到一些圆形细胞，但未观察到活细胞。少

项目成果

中山 敦, 小木會文内, 松坂賢一, 井上 孝 他3名: "ラットにおけるMTAの硬組織形成能に関する基礎的研究"日本歯科保存学雑誌. 第46巻第3号. 349-354 (2003)

Atsushi Nakayama、Fuminai Ogi、Kenichi Matsuzaka、Takashi Inoue 等 3 人：“大鼠 MTA 硬组织形成能力的基础研究”，日本保守牙科杂志，第 46 卷，第 3. 349-354 期（2003 年）。

Behaviour of bone marrow osteoblast-like cells on mineral trioxide aggregate - Morphology and expression type I collagen and bone related protein mRNAs -

骨髓成骨细胞样细胞对三氧化二矿物质聚集体的行为 - I 型胶原蛋白和骨相关蛋白 mRNA 的形态和表达 -

• 发表时间: 2005
• 期刊: International Endodontic Journal Vol.38 No.4
• 作者: A.Nakayama;B.Ogiso;N.Tanabe;O.Takeichi;K.Matsuzaka;T.Inoue
• 通讯作者: T.Inoue

ラットにおけるMTAの硬組織形成能に関する基礎的研究

MTA大鼠硬组织形成能力的基础研究

• 发表时间: 2003
• 期刊: 日本歯科保存学雑誌 46巻・3号
• 作者: 中山敦;田邊奈津子;弦間豊樹;武市収;小木曾文内;松坂賢一;井上孝
• 通讯作者: 井上孝

A Fundamental Study of the Ability of Hard Tissue Formation Using MTA in a Rat

大鼠硬组织形成能力的 MTA 基础研究

• 发表时间: 2003
• 期刊: The Japanese Journal of Conservative Dentistry Vol.46-No.3
• 作者: NAKAYAMA ATSUSHI;TANABE NATSUKO;GENMA TOYOKI;OSAMU;TAKEICHI;OGISO BUNNAI;MATSUZAKA KENICHI;INOUE TAKASHI
• 通讯作者: INOUE TAKASHI