Establishment of the periodontal ligament regeneration with mesenchymal stem cells

我们研究了生长因子对间充质干细胞（MSC）增殖和分化的影响。我们成功地在I型胶原凝胶上培养了兔骨髓间充质干细胞。bFGF对MSC的分化有抑制作用，对MSC的增殖有促进作用。TGF-β可诱导MSC向软骨细胞分化，Hyglyconan在软骨细胞外基质的合成中起重要作用。我们阐明了软骨细胞分化过程中透明质酸代谢受透明质酸合成酶（HAS 2，3）和透明质酸酶（HAYL 1，2）调节。这些酶与软骨细胞的肥大密切相关（Suzuki A.，等，Biochim Biophys Acta.，1743（1-2），2005）。（Tanimoto K.，318（2），2005）。此外，消化牙周膜周围的透明质酸可抑制牙周膜细胞的增殖，促进胶原合成。我们还研究了细胞外基质分子对牙周膜细胞增殖和分化的影响。RGD-CAP蛋白通过cDNA连接载体过量表达。siRNA用于抑制PDL上RGD-CAP蛋白的合成。最后，阐明了PDL中的钙化活性通过添加RGD-CAP而受到抑制（Doi T.，例如，口腔生物学文献，48（8），2003）。

We examined the effects of growth factors on the proliferation and differentiation of mesenchymal stem cells (MSC). We successfully cultured MSC derived from bone marrow of rabbit on type I collagen gel. The inhibition of differentiation and enhancement of proliferation of MSC was observed by addition of bFGF. Furthermore, it was suggested that MSC differentiate to chondrtocytes by addition of TGF-beta.Hyaluronan has a crucial roles in synthesis of extra cellular matrix of cartilage. We clarified that the hyaluronan metabolism during the chondrocyte differentiation is modulated by hyaluronan synthase (HAS2, 3) and hyaluronidase (HAYL 1, 2). These enzymes closely contributed to the hypestrophy of chondrocytes (Suzuki A., et al. Biochim Biophys Acta., 1743(1-2), 2005). (Tanimoto K., et al. Cell Tissue Res. 318(2), 2005). Furthermore, the digestion of hyaluronan around PDL caused the inhibition of proliferation and enhancement of collagen synthesis in PDL.We also examined the effects of extra cellular matrix molecules on the proliferation and differentiation of periodontal ligament cells (PDL). RGD-CAP protein was over-expressed by cDNA ligated vectors. The siRNA was used for suppression of RGD-CAP protein synthesis on PDL. Finally, it was clarified that activity of calcification in PDL is inhibited by addition of RGD-CAP (Doi T., et al., Arch Oral Biol., 48(8), 2003).