Regulatory mechanism for spine actin-cytoskeleton underlying maintenance of efficacy of synaptic transmission

谷氨酸对突触后神经元的作用是由树突状细胞质膜上的iGluRs介导的。iGluR在突触后特化时积累，并且通过改变突触后特化时AMPA型iGluR的数量和/或密度来改变突触功效可能与学习和记忆相关。为了研究LTP诱导的海马神经元突触后特化中AMPA和NMDA型iGluRs分布的变化，我们采用SDS-消化的FRIL，用它可以在< 20 nm分辨率，并揭示AMPA受体，但不是NMDA受体，的树突质膜和AMPA受体丰富的突触后特化的树突棘上，但不是在树突轴，调节比NMDA受体更动态LTP诱导后。

The effects of glutamate on postsynaptic neurons are mediated by iGluRs in the dendritic plasma membrane. The iGluRs are accumulated at postsynaptic specializations, and a modification of synaptic efficacy by changing number and/or density of AMPA type iGluR at the postsynaptic specialization may be relevant to learning and memory. To study LTP-induced changes in distributions of AMPA and NMDA type iGluRs in the postsynaptic specialization of hippocampal neurons, we employed SDS-digested FRIL, with which 2-dimensional distribution of the membrane proteins can be analyzed quantitatively at &lt ; 20 nm resolution, and revealed that AMPA receptors, but not NMDA receptors, are abundant on the dendritic plasma membrane and AMPA receptors at the postsynaptic specialization on the dendritic spine, but not on the dendritic shaft, are regulated more dynamically than NMDA receptors after LTP induction.