Regulatory mechanism for spine actin-cytoskeleton underlying maintenance of efficacy of synaptic transmission

脊柱肌动蛋白-细胞骨架维持突触传递功效的调节机制

• 批准号: 16500209
• 负责人: FUKAZAWA Yugo
• 金额: $ 2.37万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500209/
• 关键词: actin cytoskeleton; sipen synapses; neuronal plasticity; long-term memory; electron microscopy

The effects of glutamate on postsynaptic neurons are mediated by iGluRs in the dendritic plasma membrane. The iGluRs are accumulated at postsynaptic specializations, and a modification of synaptic efficacy by changing number and/or density of AMPA type iGluR at the postsynaptic specialization may be relevant to learning and memory. To study LTP-induced changes in distributions of AMPA and NMDA type iGluRs in the postsynaptic specialization of hippocampal neurons, we employed SDS-digested FRIL, with which 2-dimensional distribution of the membrane proteins can be analyzed quantitatively at &lt ; 20 nm resolution, and revealed that AMPA receptors, but not NMDA receptors, are abundant on the dendritic plasma membrane and AMPA receptors at the postsynaptic specialization on the dendritic spine, but not on the dendritic shaft, are regulated more dynamically than NMDA receptors after LTP induction.

谷氨酸对突触后神经元的作用是由树突状细胞质膜上的iGluRs介导的。iGluR在突触后特化时积累，并且通过改变突触后特化时AMPA型iGluR的数量和/或密度来改变突触功效可能与学习和记忆相关。为了研究LTP诱导的海马神经元突触后特化中AMPA和NMDA型iGluRs分布的变化，我们采用SDS-消化的FRIL，用它可以在< 20 nm分辨率，并揭示AMPA受体，但不是NMDA受体，的树突质膜和AMPA受体丰富的突触后特化的树突棘上，但不是在树突轴，调节比NMDA受体更动态LTP诱导后。

项目成果

Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by freeze-fracture replica labeling

• DOI: 10.1002/cne.20633
• 发表时间: 2005-08-22
• 期刊: JOURNAL OF COMPARATIVE NEUROLOGY
• 影响因子: 2.5
• 作者: Hagiwara, A;Fukazawa, Y;Shigemoto, R
• 通讯作者: Shigemoto, R

Compartment-dependent co-localization of Kir3.2-containing K^+ chnannels and GABA_B receptors in hippocampal pyramidal cells.

海马锥体细胞中含有Kir3.2的K 通道和GABA_B 受体的区室依赖性共定位。

• 发表时间: 2006
• 期刊: J.Neuroscience (In press)
• 作者: Kulik;Akos
• 通讯作者: Akos

Two-dimensional neural activity mapping of th entire population of hippocampal CA1 pyramidal cells responding to fear conditioning

海马 CA1 锥体细胞整个群体对恐惧调节的二维神经活动图谱

• 发表时间: 2005
• 期刊: Neuroscience research 51
• 作者: Inoue;Koutarou
• 通讯作者: Koutarou

Differential localization and regulation of stargazing-like protein, γ-8 and stargazing in the plasma membrane of hippocampal and cortical neurons

海马和皮质神经元质膜中观星样蛋白、γ-8和观星的差异定位和调节

• 发表时间: 2006
• 期刊: Neuroscience research (In press)
• 作者: Inamura;Mihoko
• 通讯作者: Mihoko

Immunocytochemical localization of the alpha 1A subunit of the P/Q-type calcium channel in the rat cerebellum.

大鼠小脑中 P/Q 型钙通道的 α1A 亚基的免疫细胞化学定位。

• 发表时间: 2004
• 期刊: Eur.J.Neurosci. 19
• 作者: Kulik;A.
• 通讯作者: A.