The function of the Wilms' tumor gene (WT1) overexpressed in leukemia and solid cancers

WT1基因通过两个位点的选择性剪接有4种异构体[17AA(+)KTS(+),17AA(+)KTS(-),17AA(-)KTS(+),17AA(-)KTS(-)]。在本项目中，我们证明了WT1 17AA（+）异构体[17AA(+)KTS(+),17AA(+)KTS(-)]在白血病和各种实体癌中具有抗凋亡作用，WT1 17AA(-)KTS（-）异构体促进细胞运动并在实体癌中具有抗凋亡作用。此外，使用缺失突变体的研究揭示了WT1基因的几个位点与其抗凋亡功能和细胞运动增强相关。

The WT1 gene has four isoforms by alternative splicing at two sites [17AA(+)KTS(+),17AA(+)KTS(-),17AA(-)KTS(+),17AA(-)KTS(-)]. In the present project, we have demonstrated that WT1 17AA(+) isoforms [17AA(+)KTS(+),17AA(+)KTS(-)] played antiapoptotic roles in leukemia and various kinds of solid cancers and that WT1 17AA(-)KTS(-) isoform promoted cell motility and played antiapoptotic roles in solid cancers. Moreover, the studies using deletion mutants revealed several sites of the WT1 gene associated with its antiapoptotic function and enhancement of cell motility.