Functional analysis of enhanced adaptor molecule Shc in the nuclei of the hepatocellular carcinoma

增强型接头分子Shc在肝细胞癌细胞核中的功能分析

• 批准号: 17590649
• 负责人: MASAKI Tsutomu
• 金额: $ 2.3万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590649/
• 关键词: hepatic carcinoma; adaptor molecule she; tyrosine kinase; oncogene; gastric carcinoma; 肝細胞癌

Shc protein is known to be related to cell proliferation and carcinogenesis. However, the involvement of Shc in hepatocellular carcinoma remains unknown. Surprisingly, p52 Shc was localized only in the cytoplasm of hepatocytes and cancer cells, while p46 Shc was expressed in the nuclei of normal hepatocytes and cancer cells. In addition, the expression of p46 Shc and that of p52 Shc were higher in hepatocellular carcinoma than in adjacent normal hepatocytes from the same patient. These results suggest that the enhancement of p46 Shc and p52 Shc may be related to the occurrence of hepatocellular carcinoma. Furthermore, unlike p52 Shc, p46 Shc was expressed in the nuclei of hepatocellular carcinoma cells with high proliferative activity. Therefore, p46 Shc expressed in the nuclei may play an important role in the progression of hepatocellular carcinoma. These events were also observed in colon cancer and gastric cancer.

Shc 蛋白已知与细胞增殖和癌变有关。然而，Shc 在肝细胞癌中的作用仍不清楚。令人惊讶的是，p52 Shc仅定位于肝细胞和癌细胞的细胞质中，而p46 Shc在正常肝细胞和癌细胞的细胞核中表达。此外，p46 Shc和p52 Shc在肝细胞癌中的表达高于同一患者的邻近正常肝细胞。这些结果提示p46 Shc和p52 Shc的增强可能与肝细胞癌的发生有关。此外，与p52 Shc不同，p46 Shc在具有高增殖活性的肝细胞癌细胞的细胞核中表达。因此，细胞核表达的p46 Shc可能在肝细胞癌的进展中发挥重要作用。这些事件也在结肠癌和胃癌中观察到。

项目成果

Comparison study of the expressions of myristoylated alanine-rich C kinase substrate in hepatocellular carcinoma, liver cirrhosis, chronic hepatitis, and normal liver.

肉豆蔻酰化富丙氨酸C激酶底物在肝细胞癌、肝硬化、慢性肝炎及正常肝脏中表达的比较研究。

• 发表时间: 2005
• 期刊: Int J Oncol 26
• 作者: Masaki T;Tokuda M;Yoshida S;Nakai S;16 others
• 通讯作者: 16 others

Analyses of autoantibodies against tumor-associated antigens in patients with hepatocellular carcinoma.

• DOI: 10.3892/ijo.27.4.1079
• 发表时间: 2005-10
• 期刊: International journal of oncology
• 影响因子: 5.2
• 作者: T. Himoto;S. Kuriyama;Jian-ying Zhang;E. Chan;Y. Kimura;T. Masaki;N. Uchida;M. Nishioka;E. Tan

Enhanced expression of p46 She in the nucleus and p52 She in the cytoplasm of human gastric cancer.

人胃癌细胞核中 p46 She 和细胞质中 p52 She 的表达增强。

• 发表时间: 2005
• 期刊: Int J Oncol 26
• 作者: Yukimasa S;Masaki T;Yoshida S;9 others
• 通讯作者: 9 others

Successful treatment of advanced hepatocellular carsinoma by combined administration of 5-fluorouracil and pegylated interferon-a.

5-氟尿嘧啶联合聚乙二醇干扰素-a 成功治疗晚期肝细胞癌。

• 发表时间: 2005
• 期刊: World J Gastroenterol 11
• 作者: Kurokohchi K;Takaguchi K;Kita K;Masaki T;Kuriyama S
• 通讯作者: Kuriyama S

Angiopoietin 2 displays a vascular endothelial growth factor dependent synergistic effect in hepatocellular carcinoma development in mice

• DOI: 10.1136/gut.2005.067900
• 发表时间: 2005-07
• 期刊: Gut
• 影响因子: 24.5
• 作者: H. Yoshiji;S. Kuriyama;R. Noguchi;J. Yoshii;Y. Ikenaka;K. Yanase;T. Namisaki;Mitsuteru Kitade;M. Uemura;Tsutomu Masaki;Hiroshi Fukui