Roles of chlamydial lipopoly saccharide and innate immune responses in atherogenesis

衣原体脂多糖和先天免疫反应在动脉粥样硬化形成中的作用

• 批准号: 17590763
• 负责人: ISHII Yuko
• 金额: $ 2.37万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590763/
• 关键词: Atherosclerosis; Chlamydiaceae; Lipopolysaccharide(LPS); immune response; moflonaclear cells; low density ltpoprotein(LDL); CD14; Toll-tike receptor(TLR); 肺炎クラミジア; リポ多糖; lipopolysaccharide; LDL; TLR4; LPS-binding protein; 循環器・高血圧; 生体分子; 菌体

Chlamydiaceae are small obligate intracellular parasites and classified as gram-negative bacteria. Although recent studies have suggested that chronic inflammatory reactions by Chlamydophila pneumoniae is associated with the development of atherosclerosis, little is known about the mechanisms for Chlamydiaceae -induced host cell activation.Among Chlamydiaceae-derived components, LPS is known as a potent immunomodulator, and chlamydial LPS possesses a unique lipid A structure with longer but less acyl chains. In this study, to elucidate the chlamydial LPS-induced immune responses, we evaluated the actions of chlamydial LPS on human PBMC, using Chlamydophila psittaci LPS whose structure is nearly identical to that of C. pneumoniae, and compared with those of E. coli LPS.Similar to E. coli LPS, C. psittaci LPS bound to monocytes and induced the pro-inflammatory cytokine production in an LPS-binding protein (LBP)-dependent manner. However, C. psittaci LPS was much less potent than E. coli … More LPS in both the LPS binding and cytokine production. Interestingly, the binding of C. psittaci LPS was mediated by CD14, TLR4 and CD11b, whereas the cytokine production was dependent on CD14 and TLR4 but not CD11b. Of note, ELIS A-based binding assays revealed that C. psittaci LPS directly bound to LBP and CD14; however the affinities were much less man those of E. coli LPS. Together, these observations possibly suggest that Chlamydiaceae LPS has low binding affinities for LPS-recognition molecules such as CD14 and LBP, and exhibit weak biological activities against host immune cells including monocytes, thereby contributing to the chronic (persistent) inflammatory reactions during infection. Together, these observations indicate that chlamydial LPS has the low binding affinities for LPS-recognition molecules, and exhibits weak biological activities against host immune cells such as monocytes, thereby contributing to the chronic (persistent) inflammatory reactions during chlamydial infection. Less

衣原体科是一种专性细胞内小寄生虫，属于革兰氏阴性菌。虽然最近的研究表明，肺炎衣原体的慢性炎症反应与动脉粥样硬化的发展有关，但对衣原体诱导宿主细胞活化的机制知之甚少。在衣原体衍生的组分中，LPS被认为是一种有效的免疫调节剂，衣原体LPS具有独特的脂质A结构，具有较长但较少的酰基链。为了阐明衣原体脂多糖诱导的免疫应答，我们用结构与鹦鹉热衣原体脂多糖相似的嗜衣原体脂多糖评价了衣原体脂多糖对人PBMC的作用。pneumoniae，与E. coliLPS。coliLPS、C.鹦鹉热LPS与单核细胞结合，并以LPS结合蛋白（LBP）依赖的方式诱导促炎细胞因子的产生。然而，C.鹦鹉热LPS的活性远低于E.杆菌 ...更多信息 LPS在LPS结合和细胞因子产生中的作用。有趣的是，C.鹦鹉热LPS由CD14、TLR4和CD11b介导，而细胞因子的产生依赖于CD14和TLR4，而不依赖于CD11b。值得注意的是，基于ELISA的结合试验显示C。鹦鹉热LPS与LBP和CD 14直接结合，但其亲和力远低于E. coli LPS。总之，这些观察结果可能表明衣原体LPS对LPS识别分子如CD 14和LBP具有低结合亲和力，并且对宿主免疫细胞（包括单核细胞）表现出弱的生物活性，从而导致感染期间的慢性（持续性）炎症反应。总之，这些观察结果表明衣原体LPS对LPS识别分子具有低结合亲和力，并且对宿主免疫细胞如单核细胞表现出弱的生物活性，从而导致衣原体感染期间的慢性（持续性）炎症反应。少

项目成果

Role of, Chlamydial lipopolysaccharide in innate immune responses of mononuclear phagocytes.

衣原体脂多糖在单核吞噬细胞先天免疫反应中的作用。

• 发表时间: 2005
• 作者: Tsutsumi-Ishii Y;Shimada K;Daida H;Toman R;Nagaoka I.
• 通讯作者: Nagaoka I.

エンドトキシン研究8-その新たな展開-

内毒素研究8-新进展-

• 发表时间: 2005
• 作者: 浦野貴之;尾池雄一;石井(堤) 裕子
• 通讯作者: 石井(堤) 裕子

Roles of Chlamyclophila LPS in the pathogenesis of atherosclerosis-Activation of mononuclear cells by chlamysial LPS-.

衣藻 LPS 在动脉粥样硬化发病机制中的作用 - 衣藻 LPS 激活单核细胞 -。

• 发表时间: 2006
• 期刊: Pre Symptomatic Medicine And Anti Aging 15(1)
• 作者: Tsutsumi-Ishii Y;Shimada K;Daida H;Toman R;Nagaoka I.
• 通讯作者: Nagaoka I.

Innate immune responses to chlamydial LPS, activities of chlamidial LPS and immune response in host defense.

对衣原体脂多糖的先天免疫反应、衣原体脂多糖的活性和宿主防御中的免疫反应。

• 发表时间: 2006
• 作者: Steg PG;Bhatt DL;Wilson PWF;D'Agostino R;Ohman EM;Rother J;Liau CS;Hirsch AT;Mas JL;Ikeda Y;Pencina MJ;Goto S for the REACH Registry Investigators.;Tsutsumi-Ishii Y.
• 通讯作者: Tsutsumi-Ishii Y.

動脈硬化の病態における肺炎クラミジア感染の意義-クラミジアLPSによる末梢血単核球の活性化-

肺炎衣原体感染在动脉硬化病理学中的意义 - 衣原体脂多糖激活外周血单核细胞 -

• 发表时间: 2006
• 期刊: 未病と抗老化 15
• 作者: 石井(堤) 裕子;島田 和典;他
• 通讯作者: 他