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Molecular Biologic Approach of Coronary Circulation and Coronary Endothelial Function in Patients with Cyanotic Heart Disease

紫绀型心脏病患者冠状动脉循环和冠状动脉内皮功能的分子生物学方法

基本信息

批准号：
17591091
负责人：
AKAGI Teiji
金额：
$ 1.66万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591091/
关键词：
congenial heart disease cyanosis endothelial function coronary artery oxidation stress shear stress

项目摘要

Background: Previous study demonstrated that endothelial function could be damaged by the acute hypoxia in the setting of the animal experimental model. Endothelial-derived nitric oxide and endothelial prostaglandins are released in response to the increase in shear stress due to hyperviscosity of erythrocytosis. In this regard, we hypothesized that vascular endothelial function may be damaged by long-standing cyanosis or hemodynamic stress in adult patients with cyanotic heart disease. However, little is known about endothelial function in adult patients with cyanotic heart disease. Objective: The purpose of this study was to evaluate the endothelial function and its related factors in adult patients with cyanotic heart disease. Methods: Forearm blood flow (FBF) response to handgrip exercise or brachial arterial occlusion were assessed in 14 patients with cyanotic heart disease (mean age: 26.8±6.0 years, SaO2: 82.1±8.4%) and age-matched control (mean age: 30.9±8.3 years, Sa02: 98.1±0. … More 9%) using strain gauge plethysmography. As the related factors of vascular endothelial function, red blood cell (RBC), uric acid, MDA-LDL, ICAM-1, von Willebrand factor, thrombomodulin, endothelin, erythropoietin, urine 8-hydroxy-2'-deoxyguanosine and urine NOx level were analyzed. Results: Percent change of FBF to handgrip exercise in cyanotic patients was significantly deteriorated than that in control (882.6±358.8% vs. 1428.41447.6%, p=0.007). Similarly, percent change of FBF to brachial arterial occlusion in cyanotic patients was significantly deteriorated than that in control (292.6±89.9% vs. 455.6±117.8%, p=0.002). Such percent changes were significantly correlated with oxygen saturation, RBC and ICAM-1 level. Conclusions: From these results, we concluded that vascular endothelial impairment exists in adult patients with cyanotic heart disease and closely related with severity of cyanosis or vascular. Shear stress. Such impaired endothelial function may influence on the long-term outcome in these populations. Less

背景：以往的研究表明，在动物实验模型的设置下，急性缺氧可损伤血管内皮细胞功能。内皮源性一氧化氮和内皮性前列腺素的释放是对红细胞高粘滞性引起的切应力增加的反应。在这一点上，我们假设长期存在的青紫型心脏病患者的血管内皮功能可能受到血流动力学应激的损害。然而，对青紫型心脏病成人患者的内皮功能知之甚少。目的：探讨青紫型心脏病患者血管内皮功能及其相关因素。方法：对14例青紫型心脏病患者(平均年龄：26.8±6.0岁，SaO2：82.1±8.4%)和年龄匹配的对照组(平均年龄：30.9±8.3岁，SaO2：98.1±0)进行前臂血流(FBF)检测。…超过9%)使用应变计体积描记术。分析红细胞、尿酸、丙二醛-低密度脂蛋白、细胞间黏附分子-1、血管性血友病因子、血栓调节蛋白、内皮素、促红细胞生成素、尿8-羟基-2‘-脱氧鸟苷和尿NOx水平作为血管内皮功能的相关因素。结果：青紫患者握力训练前后FBF变化率明显低于对照组(882.6±358.8%vs.1428.41447.6%，P=0.007)。与对照组相比，青紫型患者肱动脉闭塞时的空腹血流变化率明显恶化(292.6±89.9%比455.6±117.8%，P=0.002)。这种百分比变化与血氧饱和度、RBC和ICAM-1水平显著相关。结论：青紫型心脏病成人患者存在血管内皮细胞损伤，且与其严重程度或血管病变密切相关。剪切力。这种内皮功能受损可能会影响这些人群的长期预后。较少