Establish of the animal model of erythema multiforme induced by herpes simplex infection.

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We constructed the expressoin vector which contains keratin 14 promotor, human herpes simplex virus glycoprotein B (HSV-gB) and intron/ poly A signal. This construct were transfected into Hela cells and HSV-gB expression in those cells was confirmed by polyclonal antibody which was established from rabbit immunized with HSV-gB peptides.Using this construct, we are trying to generate transgenic mice which selectively express HSV-gB protein in skin. The construct were microinjected into fertilized eggs and transferred into oviduct of psuedopregnant mouse. 119 mice were obtained. We now obtaine at least 5 founders carrying the introducing HSV-gB gene detected by PCR.The expression of HSV-gB will be checked in skin and these transgenic mice will be infected with herpes simplex virus type I. We monitor the clinical sympton and survival rate. The immunological response will be measured by cytotoxic T cell response (^<51>Cr reraese assay), CD4 T cell response (^3H incorporation assay).