Establish of the animal model of erythema multiforme induced by herpes simplex infection.

单纯疱疹感染所致多形红斑动物模型的建立

• 批准号: 17591174
• 负责人: MOROI Yoichi
• 金额: $ 2.24万
• 依托单位: KYUSHU UNIVERCITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591174/
• 关键词: Herpes virus infection; Skin disease; Autoimmunity; Break down of immune trelance; 単純ヘルペスウイルス; 免疫学; 遺伝子; トランスジェニック動物

We constructed the expressoin vector which contains keratin 14 promotor, human herpes simplex virus glycoprotein B (HSV-gB) and intron/ poly A signal. This construct were transfected into Hela cells and HSV-gB expression in those cells was confirmed by polyclonal antibody which was established from rabbit immunized with HSV-gB peptides.Using this construct, we are trying to generate transgenic mice which selectively express HSV-gB protein in skin. The construct were microinjected into fertilized eggs and transferred into oviduct of psuedopregnant mouse. 119 mice were obtained. We now obtaine at least 5 founders carrying the introducing HSV-gB gene detected by PCR.The expression of HSV-gB will be checked in skin and these transgenic mice will be infected with herpes simplex virus type I. We monitor the clinical sympton and survival rate. The immunological response will be measured by cytotoxic T cell response (^<51>Cr reraese assay), CD4 T cell response (^3H incorporation assay).

我们构建了含有角蛋白14启动子、人单纯疱疹病毒糖蛋白B(HSV-GB)和内含子/聚A信号的表达载体。将此载体导入Hela细胞，用HSV-GB多肽免疫的兔建立多克隆抗体，证实HSV-GB在Hela细胞中表达，并试图利用该载体获得皮肤中选择性表达HSV-GB蛋白的转基因小鼠。将其显微注射到受精卵中，并转移到妊娠小鼠的输卵管中。共获得119只小鼠。我们现在获得至少5只携带PCR检测到的HSV-GB基因的转基因小鼠，检测HSV-GB在皮肤中的表达，并将这些转基因小鼠感染I型单纯疱疹病毒。免疫应答将通过细胞毒性T细胞应答(^&lt；51&gt；CrReraese法)、CD4T细胞应答(^~3H掺入法)进行测定。

项目成果

αヘルペスウイルスの潜伏感染と再活性化機構

α疱疹病毒的潜伏感染和再激活机制

• 发表时间: 2006
• 期刊: 日本臨床 (印刷中)
• 作者: Oka;M. 他;高橋幸博;Kaneda M;皆川洋子
• 通讯作者: 皆川洋子