Analysis of promoter regions in Hailey-Hailey disease and Darier's disease genes (2^<nd> report)

点击翻译按钮获取中文摘要

Results obtained by research in 20051) Co-transfection of ATP2C1 promoter and SP- 1 or YY- 1 clone into the cells stimulated the expression of ATP2C1.2) Expression of SP-1 itself was up regulated by raised extracellular calcium concentration in the culture of HaCat cells.3) Expression of SP- 1 was low in HHD keratinocytes and did not respond to raised extra-cellular calcium concentration in the culture due to abnormally stable-high level of calcium concentration in the cytosole of the cells caused by genetic mutation of ATP2C1.Results obtained by research in 20061) We have identified -550/-528, -488/-471, -390/1361 and -42/-20 of ATP2A2 as candidate promoter regions by the reporter assay.2) Sp 1, but not Sp3 was found to bind to -550/-528 and -488/-471 regions by gel shift assay.3) Knock down of Sp 1 protein by siRNA resulted in decrease of ATP2A2 protein in cultured normal human keratinocytes.These results implicated that controlling Sp1 expression might likely be a candidate therapeutic option for the treatment of HHD and DD.