Analysis of promoter regions in Hailey-Hailey disease and Darier's disease genes (2^<nd> report)

Hailey-Hailey 病和 Darier 病基因启动子区域分析（第 2 份报告）

• 批准号: 17591186
• 负责人: SHIGAKU Ikeda
• 金额: $ 1.79万
• 依托单位: JUNTENDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591186/
• 关键词: Hailey-Hailey disease; Darier's disease; gene; promoter; SP-1; YY-1; Sp3

Results obtained by research in 20051) Co-transfection of ATP2C1 promoter and SP- 1 or YY- 1 clone into the cells stimulated the expression of ATP2C1.2) Expression of SP-1 itself was up regulated by raised extracellular calcium concentration in the culture of HaCat cells.3) Expression of SP- 1 was low in HHD keratinocytes and did not respond to raised extra-cellular calcium concentration in the culture due to abnormally stable-high level of calcium concentration in the cytosole of the cells caused by genetic mutation of ATP2C1.Results obtained by research in 20061) We have identified -550/-528, -488/-471, -390/1361 and -42/-20 of ATP2A2 as candidate promoter regions by the reporter assay.2) Sp 1, but not Sp3 was found to bind to -550/-528 and -488/-471 regions by gel shift assay.3) Knock down of Sp 1 protein by siRNA resulted in decrease of ATP2A2 protein in cultured normal human keratinocytes.These results implicated that controlling Sp1 expression might likely be a candidate therapeutic option for the treatment of HHD and DD.

2005年的研究结果1）ATP 2C 1启动子和SP- 1或YY- 1克隆共转染入细胞刺激ATP 2C 1的表达。2）SP-1本身的表达在HaCat细胞培养物中被升高的细胞外钙浓度上调。3）SP- 1在HHD角质形成细胞中表达低，并且对升高的细胞外钙浓度没有反应。培养物中的细胞钙浓度由于ATP 2C 1基因突变引起的细胞胞浆中钙浓度异常稳定-高水平。2006年研究获得的结果1）我们已经鉴定了-550/-528，-488/-471，2）Sp1，凝胶位移分析发现Sp3与-550/-528和-488/-471区域结合，而Sp3不与之结合。用siRNA敲低Sp1蛋白可导致培养的正常人角质形成细胞中ATP 2A 2蛋白的表达减少，这些结果表明，表达可能是治疗HHD和DD的候选治疗选择。

项目成果

A case of impetigo herpetiformis associated with intrahepatic cholestasis of pregnancy

妊娠期肝内胆汁淤积疱疹样脓疱病一例

• 发表时间: 2006
• 期刊: J Dermatol 33
• 作者: Fan P;et al
• 通讯作者: et al

Clinical trial of a laser device called fractional photo-hermolysis system for acne scars

点阵光溶血系统激光设备治疗痤疮疤痕的临床试验

• 发表时间: 2006
• 期刊: J Dermatol 33
• 作者: Hasegawa T;et al
• 通讯作者: et al

A case of lymphangitic sporotrichosis occurring on both forearms with a published work review of cases of bilateral sporotrichosis in Japan.

发生在双前臂的淋巴管孢子丝菌病病例，并发表了日本双侧孢子丝菌病病例的工作回顾。

• 发表时间: 2006
• 期刊: J Dermatol 33
• 作者: Haruna K;et al
• 通讯作者: et al

Expression of DNMT-1 in patients with atopic dermatisis

特应性皮病患者DNMT-1的表达

• 发表时间: 2006
• 期刊: Arch Dermatol Res 298
• 作者: Nakamura T;Sekigawa I;Ogasawara H;Ikeda S;et al.
• 通讯作者: et al.

Superficial mucoceles of the soft palate

• DOI: 10.1159/000084769
• 发表时间: 2005-01-01
• 期刊: DERMATOLOGY
• 影响因子: 3.4
• 作者: Inoue, A;Ikeda, S;Ogawa, H
• 通讯作者: Ogawa, H