Z-Project - Bacteriophage Repository, Services and Database Construction

Z-Project - 噬菌体存储库、服务和数据库建设

• 批准号: 465136285
• 负责人: Professor Dr. Jörg Overmann
• 金额: --
• 依托单位: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/465136285?language=en
• 关键词: Project; Bacteriophage; Repository; Services; Database

The Z-Project will provide essential and central service functions for all participating groups of the SPP. It will support the individual SPP projects through state-of-the-art methodologies for determining the relevant aspects of the biology of prokaryotic viruses needed for follow-up research in the individual projects. The Z-Project will also be in charge of the long-term conservation, quality control and distribution of the isolated bioresources and their data. Besides the isolation of new viruses, the services offered comprise the determination of host spectra, growth/lysis kinetics and parameters (burst size, efficiency of plating, adsorption rate, frequency of resistance development), the analysis of phage-host interactions including receptor adsorption, replication sites, the localization of phages in biofilms using super-resolution light microscopy, and the determination of virus morphology via transmission electron microscopy. The comparative genomics of bacteriophages, their hosts, and metagenomic analyses of the viriome of environmental samples will permit a deeper characterization of virus phylogenomics and functionality. The virus-associated (meta)data will be standardized and made accessible through a central database module (PHAGODive) that will be developed using the established database BacDive as a starting point. By applying a controlled vocabulary, predefined text fields and extensive search functions, an easy, yet comprehensive, access to key information concerning the model organisms of the SPP 2330 will be offered. In addition, the previously established DSMZ-collections that currently contain about 900 different bacteriophages (many of them not yet publicly available) will serve as a unique and valuable source of reference materials for the research within the SPP. The Z-Project will be run by the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures.

Z项目将为SPP的所有参与团体提供基本的中央服务功能。它将通过最先进的方法来支持SPP的各个项目，以确定各个项目后续研究所需的原核病毒生物学的相关方面。Z项目还将负责长期保护、质量控制和分配孤立的生物资源及其数据。除了新病毒的分离外，所提供的服务还包括确定宿主谱、生长/裂解动力学和参数（爆发大小、平板接种效率、吸附速率、抗性发展频率），包括受体吸附、复制位点在内的噬菌体-宿主相互作用的分析，使用超分辨率光学显微镜在生物膜中定位噬菌体，并通过透射电子显微镜测定病毒形态。噬菌体及其宿主的比较基因组学和环境样品病毒组的宏基因组学分析将允许更深入地表征病毒的基因组学和功能。与病毒有关的（Meta）数据将标准化，并可通过一个中央数据库模块（PHAGODive）查阅，该模块将利用已建立的BacDive数据库作为起点开发。通过应用受控的词汇表、预定义的文本字段和广泛的搜索功能，将提供对关于SPP 2330的模式生物体的关键信息的简单而全面的访问。此外，先前建立的DSMZ收藏品目前包含约900种不同的噬菌体（其中许多尚未公开），将作为SPP内研究的参考材料的独特和有价值的来源。Z项目将由莱布尼茨研究所DSMZ-德国微生物和细胞培养物保藏中心负责。