Z-Project - Bacteriophage Repository, Services and Database Construction

Z项目将为SPP的所有参与小组提供基本和中心的服务功能。它将通过最先进的方法，确定个别项目后续研究所需的原核生物病毒生物学的相关方面，为个别项目提供支持。Z-Project还将负责孤立的生物资源及其数据的长期保护、质量控制和分配。除了分离新的病毒外，服务还包括确定宿主光谱、生长/裂解动力学和参数(爆发大小、平板效率、吸附速率、耐药性发展频率)、分析噬菌体与宿主的相互作用，包括受体吸附、复制位置、利用超分辨率光学显微镜定位噬菌体在生物膜中的定位，以及通过透射电子显微镜确定病毒的形态。噬菌体及其宿主的比较基因组学和环境样本病毒组的元基因组分析将使病毒系统基因组学和功能的更深层次的表征成为可能。将对与病毒有关的(元)数据进行标准化，并通过中央数据库模块(PHAGODive)进行访问，该模块将以已建立的数据库BacDive为起点进行开发。通过应用受控词汇表、预定义文本域和广泛的搜索功能，将提供对关于SPP 2330的模式生物的关键信息的简单而全面的访问。此外，先前建立的DSMZ收集目前包含约900个不同的噬菌体(其中许多尚未公开提供)，将成为SPP内研究的独特和有价值的参考材料来源。Z-项目将由莱布尼茨研究所DSMZ-德国微生物和细胞培养集合运行。

The Z-Project will provide essential and central service functions for all participating groups of the SPP. It will support the individual SPP projects through state-of-the-art methodologies for determining the relevant aspects of the biology of prokaryotic viruses needed for follow-up research in the individual projects. The Z-Project will also be in charge of the long-term conservation, quality control and distribution of the isolated bioresources and their data. Besides the isolation of new viruses, the services offered comprise the determination of host spectra, growth/lysis kinetics and parameters (burst size, efficiency of plating, adsorption rate, frequency of resistance development), the analysis of phage-host interactions including receptor adsorption, replication sites, the localization of phages in biofilms using super-resolution light microscopy, and the determination of virus morphology via transmission electron microscopy. The comparative genomics of bacteriophages, their hosts, and metagenomic analyses of the viriome of environmental samples will permit a deeper characterization of virus phylogenomics and functionality. The virus-associated (meta)data will be standardized and made accessible through a central database module (PHAGODive) that will be developed using the established database BacDive as a starting point. By applying a controlled vocabulary, predefined text fields and extensive search functions, an easy, yet comprehensive, access to key information concerning the model organisms of the SPP 2330 will be offered. In addition, the previously established DSMZ-collections that currently contain about 900 different bacteriophages (many of them not yet publicly available) will serve as a unique and valuable source of reference materials for the research within the SPP. The Z-Project will be run by the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures.