Analysis of morphogenesis signal system in the thymic tumor tissues and its application to myasthenia gravis treatment

胸腺肿瘤组织形态发生信号系统分析及其在重症肌无力治疗中的应用

• 批准号: 17591340
• 负责人: KOJIMA Masayuki
• 金额: $ 2.18万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591340/
• 关键词: Hedgehog signal; Thymus; THymoma; Thymic carcinoma; Myasthenia gravis; Shh; Gli; Hedgehog signal; 形態形成シグナル

During this study period, we performed Hedgehog (Hh) signaling system analysis in 45 thymus tissues and reported analytical results about 26 specimens (Anticancer Research 25:00 3697-3702,2005). Summary of results obtained during the study period is as follows.1. We analyzed for the first time Hh signaling system in the adult thymus tissue. As a result, Shh was frequently expressed in the adult normal thymus, but Glil, which is a marker of the activation of Hh signaling pathway, was not expressed.2. Interestingly, Shh expression showed an inverse relationship to histological classification of WHO in the thymoma. Namely, Shh expression rate was 100% in type A,91.6% in type AB,62.5% in type Bl,25% in type B2,and 0% in type C. Glil expression was 100% in type A,36.4% in type AB,75% in type B1,25% in type B2,and 100% in type C. Thus, Glil expression was significantly higher in the thymoma than in the normal thymus (P<0.0001), suggesting Hh pathway activation in the thymoma.3. Because Shh expression was hardly found in one thymic carcinoma, in which a marked Glil expression was found, ligand-independent activation was suggested in this case.4. Twelve of the 26 cases were myasthenia gravis cases, but were not able to find significant relations between myasthenia gravis and Hh signal activation.5. The establishment of the thymoma cell line did not succeed.

在本研究期间，我们在45个胸腺组织中进行了刺猬（HH）信号系统分析，并报告了大约26个标本的分析结果（抗癌研究25:00 3697-3702,2005）。研究期间获得的结果摘要如下1。我们首次在成年胸腺组织中分析了HH信号系统。结果，SHH经常在成年正常胸腺中表达，但是Glil（这是HH信号通路激活的标志）的GLIL未表达。2。有趣的是，SHH表达与胸腺瘤中的组织学分类呈反比关系。即，A型的SHH表达速率为100％，AB型为91.6％，BL型为62.5％，B2型为25％，C型为0％，A型的Glil表达为100％，AB型为36.4％，B2型B1型为B1型，B1型为1.45％。因此，Glil表达在P Themoma中，PLIL表达在Plil <themsape <themsape <0. 000均高于<thems <thems <thems <thems <thems的正常状态（p。胸腺瘤中的途径激活3。由于在一种胸腺癌中几乎没有发现SHH表达，其中发现了明显的神经胶质表达，因此在这种情况下提出了不依赖配体的非依赖性激活。4。在26例病例中，有十二例是肌无力的重症病例，但无法在肌动脉症和HH信号激活之间找到显着关系。5。胸腺瘤细胞系的建立没有成功。

项目成果

Immunohistochemical staining of Hedgehog pathway-related proteins in human thymomas.

人胸腺瘤中 Hedgehog 通路相关蛋白的免疫组织化学染色。

• 发表时间: 2005
• 期刊: Anticancer Research 25
• 作者: Tasaki A;Akiyoshi T;Koga K;Nakashima H;Yamanaka N;Kubo M;Matsumoto K;Kojima M;Tanaka M Nakamura M;Katano M
• 通讯作者: Katano M

Immunohistochemical staining of Hedgehog pathway-related proteins in human tymomas

人鼓室瘤中 Hedgehog 通路相关蛋白的免疫组织化学染色

• 发表时间: 2005
• 期刊: Anticancer Research 25
• 作者: Kim R;et al.;Tasaki A. et al.
• 通讯作者: Tasaki A. et al.

Immunohistochemical staining of Hedgehog pathway-related proteins in human thymomas

人胸腺瘤中 Hedgehog 通路相关蛋白的免疫组织化学染色

• 发表时间: 2005
• 期刊: Anticancer Research 25
• 作者: Tasaki A;Akiyoshi T;Koga K;Nakashima H;Yamanaka N;Kubo M;Matsumoto K;Kojima M;Tanaka M Nakamura M;Katano M;Deitch EA;Kim R.;Tasaki A. et al.
• 通讯作者: Tasaki A. et al.