Analysis of molecular genetics to the bone resorption mechanism that an immune cell causes

（An experiment method）Manufacturing of the animal experiment model who used 1） FK506 ： I did intraperitoneal injection of FK506 to an experimental group with isotonic sodium chloride solution in control group with an ICR maleness mouse of an instar day after day for four weeks for six weeks。2）形式观察：有一条线通过免疫病理学消除，我做了一个乳头状瘤移植，并观察B细胞。我比较了一个TRAP死亡图像对应于下一个和观察到的控制组，以及每个实验组的变化。Furthermore，一个大型的intestine组织，一个组织产生了移植和表演的历史观察。B细胞和骨细胞的实验室关系：骨细胞聚集骨髓瘤的形成和第一个骨细胞样细胞和添加活性形式维生素D。我挑选了一个ICR-maleness小鼠的一个安装7周的中间淋巴节点，我分配了一个CD 45 R阳性细胞并收集了它。 ... More 在接触目录或接触中添加了B细胞和B细胞，我使用了一个过滤器来测试一个共存培养系统，其中一些因素推动了骨质疏松症的形成，并通过骨质疏松症的形成间接和评估了它。4）我收集了一个骨髓瘤，超过了一个tibia，我完成了陷阱死亡，在剂量之后，观察了一个对古代葡萄糖数量的测量，十天后文化的差异性。5）我挑选了一个FK剂量小鼠的中间淋巴节点（MLN），并在FACS.6）我在Bio-Plex中测量血清细胞因子。（一个结果/假设）与免疫组织化学消亡的图像·控制组和TRAP消亡的图像对应， B细胞一般打开，以及一个最后一个TRAP阳性的状态和旧的，我累积了未被识别的。However，有一个实验组，在骨骼表面上有一个旧的TRAP阳性，并在与控制组的比较中增加。当我试着把两个都放在一起的时候，B细胞似乎在累积它附近。根据一个文化实验，我比较了接触群体和非接触群体，由一个带有TRAP阳性细胞的骨细胞组成的数量组成，而带有TRAP阳性的许多核细胞的骨细胞则增加了许多核细胞。就像Interleukin-6和TNF-α一样，我在控制组中显示了一个低价值，并在只有FK 506管理的人群中显示了一个高价格。在另一个词中，有人建议说炎症细胞因子比FK 506的控制水平更高。CD4+CD45RBloCD25+ （Treg） decreased in a FK506 treated group more significantly than control group。A tendency to increase had CD4+ CD45RBhiCD25-than control group in a FK50G treated group。Less（低）

(An experiment method) Manufacture of the animal experiment model who used 1) FK506 : I did intraperitoneal injection of FK506 to an experimental group with isotonic sodium chloride solution in control group with an ICR maleness mouse of an instar day after day for four weeks for six weeks. 2) form observation : There was a line by immunohistochemistry dyeing I made a paraffin graft of a tibia, and to observe B cell. I compared a TRAP dyeing image corresponding to it next and observed control group and a change in an experimental group each. Furthermore, a large intestine organization produced an organization graft and performed histologic observation. 3) A culture experiment to examine relations of B cell and osteoclast : The formation of osteoclast gathered a myeloblast and first osteogenetic cell-like cell and added active form vitamin D. I picked out a mesentery lymph node of an ICR maleness mouse of an instar for seven weeks and I divided a CD45R positive cell and collected it. I … More added B cell and B cell came in contact directly or and I used a filter for coexistence culture system to examine whether some factors promoted the formation of osteoclast indirectly and evaluated it by the formation of osteoclast. 4) I gathered a myeloblast than a tibia, the thighbone and I performed TRAP dyeing and, after the dosage, observed a measurement of the number of the osteoclast, form differentiation ability after culture of ten days. 5) I picked out mesentery lymph node (MLN) of an FK dosage mouse and analyzed it in FACS.6) I measured serum cytokine in Bio-Plex.(A result / consideration) With an immunohistochemistry dyeing image of・control group and a TRAP dyeing image corresponding to it, B cell opened generally, and a state and osteoclast of TRAP positive which I accumulated were not recognized. However, with an experimental group, there was osteoclast of TRAP positive on the surface of a bone and increased in comparison with control group. The B cell seemed to accumulate it near osteoclast of TRAP positive when I tried to put both together. By a・culture experiment, I compared contact group with non-contact group with the number of the formation of an osteoclast of many nucleuses-like cell with TRAP positive, and osteoclast cells of many nucleuses increased with TRAP positive significantly. As well as・interleukin-6 and TNF-α, I showed a low value in control group and showed a high price in the crowd who administered only FK506. In other words it was suggested that inflammatory cytokine increased than control by FK506.・CD4+CD45RBloCD25+ (Treg) decreased in a FK506 treated group more significantly than control group. A tendency to increase had CD4+CD45RBhiCD25-than control group in a FK50G treated group. Less