Experimental study about bone regeneration by growth factor addition bone substitute materials

近年来，牙种植体在恢复咬合功能方面得到了应用。目的：研究骨形态发生蛋白和碱性成纤维细胞生长因子复合β-磷酸三钙(β-TcP)作为体内可吸收骨替代材料在体内扩散腔(DC)中的成骨效果，以期开发出一种具有一致成骨能力的骨替代材料，用于临床应用。材料和方法：制备6组DC：a；β-Tcp+等渗氯化钠溶液，b；13-Tcp+bFGF1μg，c；β-Tcp+bFGF10μg，d-；β-Tcp+BMP10μg，e；β-Tcp+bFGF1pg+BMP10μg，f；β-Tcp+bFGF10μg+BM.P 10μg。分别于2、3、4、8、16、24周取材，通过组织学和生化指标分析成骨能力。结果：从第2周开始，组织学观察到含有rh-BMP的新生骨形成，3周后可见活跃的新骨形成。“d”组在DC膜外与直接生物体接触部位的骨新生明显。结论：在本实验模型中，先加入rh-BMP(0.5μg/μ1)，然后加入低浓度(0.05μg/μ1)的碱性磷酸酶(rh-BMP)和碱性成纤维细胞生长因子(bFGF0.0 5μg/μ1)，即可获得最佳的成骨条件。

In late years dental implantation becomes apply in recovery of an occlusion function, and. reconstruction of alveolar bone of that purpose holds the important role.Purpose:In this study, we examined the efficacy of bone morphogenetic protein ( rh-BMP ) and basic fibroblast growth factor ( bFGF ) incorporated β-tricalcium phosphate ( β-TCP ) 'which was an absorbable bone substitute material in vivo on ontogenesis in a diffusion chamber ( DC ) to develop a bone substitute having consistent osteogenic capability for clinical applications. Materials and methods:We prepared six groups of DCs: a;β-TcP + isotonic sodium chloride solution, b;13-TCP +bFGF 1 μg, c;β-TCP +bFGF 10μg, d-;β-TCP +BMP 10μg, e;β-TCP +bFGF.1 pg +BMP 1Oμg, f;β-TCP +bFGF 10μg +BM.P 10μg. The prepared solutions were injected into DCs, which were subcutaneously implanted into the backs of F344 rats. DCs were harvested after 2,3,4,8,16 or 24 weeks and analyzed for bone forming capability by determining histological and biochemical markers.Result:We observed the formation of de novo bone in their groups to contain rh-BMP histologically since the second week, and the formation of active new bone was noted since three weeks. In the particularly bone neogenesis in the part which contacted with direct organism on the membrane outside of DCs was remarkable in the group "d". The alkaline phosphatase activity and osteocalcine content in the group of "d" and "e" were significantly higher than other groups at any time.Conclusion:It is suitable for an optimum condition in bone neogenesis in this study model, which added rh-BMP (0.5 μg/μ1) incorporated β-TCP and then bFGF of low concentration (0.05 μg/μ1).