Experimental study about bone regeneration by growth factor addition bone substitute materials

近年来，种植牙在恢复牙合功能方面的应用越来越广泛。牙槽骨的重建具有重要的作用。目的：在本研究中，我们研究了骨形态发生蛋白（rh-BMP）和碱性成纤维细胞生长因子（bFGF）结合可吸收的骨替代材料β-磷酸三钙（β-TCP）在体内扩散室（DC）中对个体发生的影响，以开发具有一致成骨能力的骨替代物用于临床应用。材料和方法：制备了6组dc: a；β-TcP +等渗氯化钠溶液，b；13-TCP +bFGF 1 μg, c；β-TCP +bFGF 10μg, d-；β-TCP +BMP 10μg, e；βtcp + bFGF。1 pg +BMP 10 μg, f；β-TCP +bFGF 10μg +BM。P 10μg。将制备的溶液注射到dc中，dc皮下植入F344大鼠背部。在2、3、4、8、16或24周后采集树突细胞，通过测定组织学和生化指标分析其成骨能力。结果：2周后组织学上观察到含rh-BMP组新生骨的形成，3周后观察到活性新生骨的形成。特别是与树突状细胞外膜直接接触的部分骨新生在“d”组中表现显著。“d”和“e”组的碱性磷酸酶活性和骨钙素含量在任何时候都显著高于其他各组。结论：加入rh-BMP （0.5 μg/μ1）结合β-TCP，再加入低浓度bFGF (0.05 μg/μ1)，是本研究模型骨新生的最佳条件。

In late years dental implantation becomes apply in recovery of an occlusion function, and. reconstruction of alveolar bone of that purpose holds the important role.Purpose:In this study, we examined the efficacy of bone morphogenetic protein ( rh-BMP ) and basic fibroblast growth factor ( bFGF ) incorporated β-tricalcium phosphate ( β-TCP ) 'which was an absorbable bone substitute material in vivo on ontogenesis in a diffusion chamber ( DC ) to develop a bone substitute having consistent osteogenic capability for clinical applications. Materials and methods:We prepared six groups of DCs: a;β-TcP + isotonic sodium chloride solution, b;13-TCP +bFGF 1 μg, c;β-TCP +bFGF 10μg, d-;β-TCP +BMP 10μg, e;β-TCP +bFGF.1 pg +BMP 1Oμg, f;β-TCP +bFGF 10μg +BM.P 10μg. The prepared solutions were injected into DCs, which were subcutaneously implanted into the backs of F344 rats. DCs were harvested after 2,3,4,8,16 or 24 weeks and analyzed for bone forming capability by determining histological and biochemical markers.Result:We observed the formation of de novo bone in their groups to contain rh-BMP histologically since the second week, and the formation of active new bone was noted since three weeks. In the particularly bone neogenesis in the part which contacted with direct organism on the membrane outside of DCs was remarkable in the group "d". The alkaline phosphatase activity and osteocalcine content in the group of "d" and "e" were significantly higher than other groups at any time.Conclusion:It is suitable for an optimum condition in bone neogenesis in this study model, which added rh-BMP (0.5 μg/μ1) incorporated β-TCP and then bFGF of low concentration (0.05 μg/μ1).