Experimental study about bone regeneration by growth factor addition bone substitute materials

近年来，种植牙逐渐被应用于咬合功能的恢复。目的：本研究旨在探讨骨形态发生蛋白（bone morphogenetic protein，rh-BMP）和碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）复合β-磷酸三钙（β-tricalcium phosphate，β-TCP）作为一种可吸收的骨替代材料，在扩散室（diffusion chamber，DC）中对骨形成的影响，以开发一种具有稳定成骨能力的骨替代材料。材料与方法：制备6组DCs：a;β-TcP +等渗氯化钠溶液，B;13-TCP +bFGF 1 μg，c;β-TCP +bFGF 10μg，d-;β-TCP +BMP 10 μg，e;β-TCP + bFGF. 1 pg +BMP 10 μg，f;β-TCP +bFGF. 10μg +BM.P 10μg。将制备的溶液注射到DC中，将DC皮下植入F344大鼠背部。结果：rh-BMP组和rh-BMP组从第2周开始组织学观察到新生骨形成，第3周开始观察到活性新骨形成。特别是在DCs外膜上与直接生物接触的部位，d组的骨新生明显。结论：在本实验模型中，先加入rh-BMP（0.5 μg/μ l），再加入β-TCP，再加入低浓度bFGF（0.05 μ g/μ l），是一种较理想的骨再生条件。

In late years dental implantation becomes apply in recovery of an occlusion function, and. reconstruction of alveolar bone of that purpose holds the important role.Purpose:In this study, we examined the efficacy of bone morphogenetic protein ( rh-BMP ) and basic fibroblast growth factor ( bFGF ) incorporated β-tricalcium phosphate ( β-TCP ) 'which was an absorbable bone substitute material in vivo on ontogenesis in a diffusion chamber ( DC ) to develop a bone substitute having consistent osteogenic capability for clinical applications. Materials and methods:We prepared six groups of DCs: a;β-TcP + isotonic sodium chloride solution, b;13-TCP +bFGF 1 μg, c;β-TCP +bFGF 10μg, d-;β-TCP +BMP 10μg, e;β-TCP +bFGF.1 pg +BMP 1Oμg, f;β-TCP +bFGF 10μg +BM.P 10μg. The prepared solutions were injected into DCs, which were subcutaneously implanted into the backs of F344 rats. DCs were harvested after 2,3,4,8,16 or 24 weeks and analyzed for bone forming capability by determining histological and biochemical markers.Result:We observed the formation of de novo bone in their groups to contain rh-BMP histologically since the second week, and the formation of active new bone was noted since three weeks. In the particularly bone neogenesis in the part which contacted with direct organism on the membrane outside of DCs was remarkable in the group "d". The alkaline phosphatase activity and osteocalcine content in the group of "d" and "e" were significantly higher than other groups at any time.Conclusion:It is suitable for an optimum condition in bone neogenesis in this study model, which added rh-BMP (0.5 μg/μ1) incorporated β-TCP and then bFGF of low concentration (0.05 μg/μ1).