Development of novel therapy for TMJ arthritis by Gene transfer using with sonoporation combined with microbubble-based ultrasound contrast agent

通过基因转移结合声孔结合微泡超声造影剂开发治疗颞下颌关节炎的新疗法

• 批准号: 17592097
• 负责人: TOMINAGA Kazuhiro
• 金额: $ 2.24万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592097/
• 关键词: temporomandibular disorder; ultrasonographic gene induction; interleukin-1; IL-1 receptor antagonist; svnovial cells; microbubble; PGE2; anti-inflammatory effect; アジュバント関節炎; IL1-ra

Administration of exogenous IL-1 receptor antagonist (IL-1ra) is effective in reducing the inflammatory reactions mediated by IL-1. In the present study, we transfected with a plasmid containing IL-1ra gene into synovial cells by the sonoporation method, and investigated the effect of IL-1ra gene transfection on inflammatory reactions induced by lipopolysaccharide (LPS) in vitro. IL-1ra- transfected synovial cells produced the extracellular IL-lra, and its level increased remarkably when the cells were cultured with LPS for 48 h. Interestingly, treatment of LPS enhanced the release of IL-1β in the culture supernatant of IL-1ra-transfected cells, however, a significant decrease of PGE2 release was observed when the cells were cultured with LPS for 60 h. These results indicate that IL-1ra could play a valuable regulatory role in inflammatory reactions of synovial cells, suggesting that the administration of IL-1ra gene with sonoporation is a useful method for the control of inflammation in arthritis.

外源性IL-1受体拮抗剂（IL-1 ra）可有效减轻IL-1介导的炎症反应。本研究采用声孔法将IL-1 ra基因转染滑膜细胞，观察IL-1 ra基因转染对脂多糖（LPS）诱导的滑膜细胞炎症反应的影响。转染IL-1 ra的滑膜细胞产生胞外IL-1 ra，与LPS共同培养48 h后，其分泌量显著增加。LPS处理可促进IL-1 ra转染细胞培养上清中IL-1β的释放，但LPS处理60 h后，PGE 2的释放明显减少。这些结果表明，IL-1 ra可以发挥有价值的调节作用，滑膜细胞的炎症反应，这表明，IL-1 ra基因的管理与声孔是一个有用的方法，用于控制炎症关节炎。

项目成果

Relationship between cortical bone formation on mandibular condyles and alternation of the magnetic resonance signals characteristic during growth

下颌骨髁皮质骨形成与生长过程中磁共振信号特征变化的关系

• 发表时间: 2007
• 期刊: Am J Orthod Dentofacial Orthop 131
• 作者: Morimoto;Y.
• 通讯作者: Y.

Changes of Temporomandibular Disk Position during growth in young Japanese

日本年轻人成长过程中颞下颌盘位置的变化

• 发表时间: 2007
• 期刊: Dentomaxillofac Radiol 36
• 作者: Tominaga;K.
• 通讯作者: K.

Relationship between TNF-αandTUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint.

兔颞下颌关节关节炎中TNF-α与TUNEL阳性软骨细胞的关系

• 发表时间: 2006
• 期刊: J Oral Pathol Med 35
• 作者: Hirota;Y.;Habu;M.;Tominaga;K.;Sukedai;M.;Matsukawa;A.;Nishihara;T.,Fukuda,J.
• 通讯作者: T.,Fukuda,J.

Three-dimensional identification of hemangiomas and feeding arteries in the head and neck region using combined phase-contrast MR angiography and fast asymmetric spin-echo sequences

• DOI: 10.1016/j.tripleo.2005.06.009
• 发表时间: 2005-11-01
• 期刊: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
• 作者: Tanaka, T;Morimoto, Y;Ohba, T
• 通讯作者: Ohba, T

The morphological features of the cartilage during distraction osteogenesis in rabbit mandible.

兔下颌骨牵张成骨过程中软骨的形态特征。

• 发表时间: 2005
• 期刊: Dent Jpn 41
• 作者: Furuta;N.
• 通讯作者: N.