Radical Production and Induction of Apoptosis for Phenolic Antioxidants

酚类抗氧化剂的自由基产生和细胞凋亡诱导

基本信息

批准号：
17592104
负责人：
OKADA Norihisa
金额：
$ 1.6万
依托单位：
Meikai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592104/
关键词：
Phenol Antioxidant Radical Apoptosis ユージノールイソユージノール

项目摘要

Eugenol (EUG) is used as ZOE cements in dentistry. EUG and its isomer (IsoEUG) generate reactive oxygen species (ROS) by photooxidation. We investigated cytotoxic effects of visible light (VL) irradiated EUG and IsoEUG on oral mucous membranes of mice. Tissue damage in IsoEUG samples was markedly greater than in EUG samples even without VL-irradiation and the potency of toxicity for both compounds was further enhanced by VL irradiation. Histopathological examination using H＆E stain and TUNEL method suggested that the biological event characterized was predominantly a necrosis, not an apoptosis.Next, we investigated cytotoxic effects photoinitiators, camphorquinone (CQ) and 9 fluorenone (9F) on oral mucosa of mice. The buccal epithelium treated with CQ showed marked thickening of pricle cell layer due to cellular edema, indicating acute inflammation. Similar observations were found in 9F. VL-irradiated CQ and 9F showed much more severe inflammation than non-irradiated ones. The induction of apoptosis was not found in CQ and 9F with and without VL irradiation. To evaluate the protective effects of phenolic compounds such as vitamin E the radical-scavenging activity of alpha-tocopherol (TOC), beta-TOC, gamma-TOC and delta-TOC was investigated using the induction period method. Alkyl and peroxy radicals were scavenged by these tocopherols. Oxidative stress by photoinitiators and eugenol-related compounds with VL irradiation may be suppressed by co-antioxidants tocopherols, glutathione, ascorbates in vivo.

Eugenol（EUG）用作牙科中的Zoe水泥。 EUG及其异构体（Isoeug）通过光氧化产生活性氧（ROS）。我们研究了可见光（VL）辐照的EUG和ISOEUG对小鼠口服粘膜的细胞毒性作用。即使没有VL辐照，ISOEUG样品中的组织损伤也明显大于EUG样品，并且通过VL辐射进一步增强了两种化合物的毒性。使用H＆E染色和TUNEL方法的组织病理学检查表明，所表征的生物学事件主要是坏死，而不是凋亡。我们研究了细胞毒性效应光接头，樟脑喹酮（CQ）（CQ）和9 F氟酮（9F）对小鼠的口服Mucosa。用CQ处理的颊上皮因细胞水肿而标记的原代细胞层增厚，表明急性炎症。在9F中发现了类似的观察结果。 VL辐射的CQ和9F的感染比未辐照的感染更为严重。在CQ和9F中未发现和没有VL辐照的9F诱导凋亡。为了评估酚类化合物（例如维生素E）的受保护作用，使用感应期方法研究了α-生育酚（TOC），β-TOC，γ-TOC和Delta-TOC的自由基扫描活性。这些生育酚清除了烷基和过氧自由基。光剂和Eugenol相关化合物的氧化应激可能会因抗抗氧化剂的生育酚，谷胱甘肽，抗逆性，在体内抑制。