Establishment of database of IFN-α production test and risk management of hepatocellular carcinoma in patients with hepatitis C virus.

丙型肝炎病毒患者IFN-α产生检测及肝细胞癌风险管理数据库的建立

• 批准号: 17606005
• 负责人: UNO Kazuko
• 金额: $ 2.24万
• 依托单位: Louis Pasteur Center for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17606005/
• 关键词: IFN-a production; Hepatitis C virus infection; Hepatocellular carcinoma; Cancer Risk; Plasmacytoid dendritic cell

Previously, we published a manuscript entitled "Impairment of IFN-α production capacity in patients with hepatitis C virus and the risk of the development of hepatocellular carcinoma" in World J Gastroenterology, which forms the basis of this project. The project entailed the establishment of a database of IFN-α production and blood tests spanning 17 years by the Louis Pasteur Center for Medical Research. Data for IFN-α production was obtained retrospectively over a 17-year period by examining medical records in a study population of 2,315 individuals, of which 112 healthy controls and 20 HCV-infected patients were selected. Sixty percent of the HCV-infected patients had impaired or declining IFN-α production in comparison to 17% in the healthy control group. Mean IFN-α levels were lower in patients who developed hepatocellular carcinoma than in the HCV-infected patients who remained cancer-free. These results have been published in J IFN ＆ Cytokine Research as "Impaired interferon-a production and the risk of cancer development." Further, we established the methodto detect IFN-α producing cells by Sendai virus stimulation using FACS, and the main IFN-α producing cells were plasmacytoid dendritic cells. This method will be useful to understand the analysis of impaired IFN-α production.

此前，我们在World J Gastroenterology上发表了一篇题为“丙型肝炎病毒患者IFN-α产生能力的损害和肝细胞癌发展的风险”的手稿，这是该项目的基础。该项目要求路易斯·巴斯德医学研究中心建立一个17年的IFN-α生产和血液检测数据库。IFN-α产生的数据是通过检查2，315人的研究人群的医疗记录回顾性获得的，其中选择了112名健康对照和20名HCV感染患者。60%的HCV感染患者IFN-α的产生受损或下降，而健康对照组为17%。发生肝细胞癌的患者的平均IFN-α水平低于未发生癌症的HCV感染患者。这些结果已发表在J IFN & Cytokine Research上，标题为“干扰素-a生产受损与癌症发展风险”。“此外，我们建立了用流式细胞仪检测仙台病毒刺激产生IFN-α的细胞的方法，主要产生IFN-α的细胞是浆细胞样树突状细胞。这种方法将有助于理解IFN-α产生受损的分析。

项目成果

Impaired interferon-α production and the risk of cancer development

干扰素-α 产生受损和患癌症的风险

• 期刊: J IFN ＆ Cytokine Res. (In press)
• 作者: Kazuko Uno;Mayumi Hirosaki;Kazuhiro Kakimi;Masumi Tominaga;Yoshiki Suginoshita;Goji Hasegawa;Michiaki Fukui;Naoto Nakamura;Taro Shirakawa;Tsunataro Kishida
• 通讯作者: Tsunataro Kishida

Impaired IFN-α production capacity and the risk of cancer

IFN-α 产生能力受损和患癌症的风险

• 发表时间: 2005
• 期刊: Cell Res Vol. 15, No. 10
• 作者: Uno K;Hirosaki M;Kakimi K;Tominaga M;Hasegawa G;Suginoshita Y;Fukui M;Nakamura N;Shirakawa T;Kishida T
• 通讯作者: Kishida T

Pleiotropic interferon functions

多效性干扰素功能

• 发表时间: 2006
• 期刊: Nippon Rinsho vol.64, No.7
• 作者: ISOZAKI;Hatsuhito;Uno K
• 通讯作者: Uno K

Clinical utility of 2',5'-oligoadenylate synthetase activity measurement : Using whole blood as a highly sensitive method to detect the effects of IFN

2,5-寡腺苷酸合成酶活性测量的临床应用：使用全血作为检测 IFN 效果的高灵敏度方法

• 发表时间: 2006
• 期刊: J Virol. Methods 136
• 作者: Uno K;Suginoshita Y;Kakimi K;Moriyasu F;Nakano K;Nakamura N;Fujita T;Horino Y;Sato T;Kishida T
• 通讯作者: Kishida T

MPO-ANCA関連血管炎急性期のサイトカイン動態と大量ガンマグロブリン(IVIg)療法による変動.

MPO-ANCA 相关血管炎急性期的细胞因子动态以及高剂量丙种球蛋白 (IVIg) 治疗引起的变化。

• 发表时间: 2006
• 期刊: Pasken Journal 2005 18
• 作者: Liu N;Shimizu S;Ito-Ihara T;et al.;猪原登志子ら;猪原登志子ら
• 通讯作者: 猪原登志子ら