Dissecting the molecular interplay between tumor cells and stroma in primary liver cancer (B08)

剖析原发性肝癌中肿瘤细胞和基质之间的分子相互作用（B08）

• 批准号: 466472154
• 金额: --
• 依托单位: Pathologisches Institut
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/466472154?language=en
• 关键词: Dissecting; molecular; interplay; between; tumor

Project B08 (Longerich/Pellegrino; Dissecting the molecular interplay between tumor cells and stroma in primary liver cancer) has entered the SFB/TR 209 in late 2018 and analyses mechanisms underlying plasticity of liver cancer cells, which bear on tumor differentiation and therapy resistance. The project has identified candidate genes affecting liver cancer cell differentiation/plasticity by acting on tumor cells and/or tumor-associated stroma using exome/transcriptome sequencing of different liver cancer variants. In the new funding period the gain-of-function phenotypic driver candidates will be evaluated in mosaic mouse models and functionally characterized in vitro and in vivo with the aim to identify modes of interdependency between tumor cell plasticity and tumor-associated stroma and potential ways of intervention.

项目B 08（Longerich/Pellegrino;剖析原发性肝癌中肿瘤细胞和基质之间的分子相互作用）已于2018年底进入SFB/TR 209，并分析了肝癌细胞可塑性的潜在机制，这些机制与肿瘤分化和治疗抵抗有关。该项目通过使用不同肝癌变体的外显子组/转录组测序，确定了影响肝癌细胞分化/可塑性的候选基因，这些基因作用于肿瘤细胞和/或肿瘤相关基质。在新的资助期内，将在嵌合小鼠模型中评估功能获得表型驱动候选物，并在体外和体内进行功能表征，旨在确定肿瘤细胞可塑性和肿瘤相关基质之间的相互依赖模式以及潜在的干预方式。