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Charactrization of gene for metabolic pathway that is responsible for germination and growth of rice under submerged conditions.

负责水稻在水下条件下发芽和生长的代谢途径基因的表征。

基本信息

批准号：
14560002
负责人：
NAKAZONO Mikiko
金额：
$ 2.3万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560002/
关键词：
rice submerged conditions growth aldehyde dehydrogenase acetyl-CoA synthetase 湛水条件

项目摘要

Rice is able to germinate in a waterlogged, oxygen-deficient environment. When rice seeds are germinated under submerged conditions, only a coleoptile (an organ that has a cylindrical sheath-like form) elongates to lengths of 5-10 cm, and the growth of the foliage leaf primordial of the seminal root does not follow. It is known that the ethanolic fermentation is necessary for efficient elongation of the coleoptiles. In this study, we found that the pathway for conversion of acetaldehyde to acetyl-CoA is also important for the coleoptile elongation. Aldehyde dehydrogenase 2 (ALDH2) and acetyl-CoA synthetase (ACS) are responsible for the pathway. At last one gene, ALDH2a and ACSI, is highly expressed in coleoptiles under submerged conditions. Transgenic rice plants, in which antisense ALDH2a ACSI gene is expressed, were generated. Using the transgenic plats, we will examine whether ALDH2 and ACS affect germination of seeds and elongation of coleoptiles under submerged conditions in the near future.

水稻能够在积水、缺氧的环境中发芽。当水稻种子在浸没条件下发芽时，只有胚芽鞘（一种具有圆柱形鞘状形式的器官）伸长至5-10厘米的长度，并且种子根的叶原基的生长不会随之而来。已知乙醇发酵对于胚芽鞘的有效伸长是必需的。在这项研究中，我们发现乙醛转化为乙酰辅酶A的途径对胚芽鞘的伸长也很重要。醛脱氢酶2（ALDH 2）和乙酰辅酶A合成酶（ACS）负责该途径。最后一个基因ALDH 2a和ACSI在淹水条件下在胚芽鞘中高度表达。获得了表达反义ALDH 2a ACSI基因的转基因水稻植株。利用这些转基因植株，我们将在不久的将来研究ALDH 2和ACS是否影响种子的萌发和胚芽鞘在淹水条件下的伸长。