Studies on Structures and Specific Functions in Bioinoganic Chemistry

生物化学中的结构和特定功能研究

• 批准号: 04225101
• 负责人: YAMAUCHI Osamu
• 金额: $ 21.06万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04225101/
• 关键词: Biological metal complex; Multiple interaction; Biological reaction process; Metalloenzyme model; Molecular recognition system; Oxygen carrier; Election transfer pathway; 人工変異タンパク質; 国際シンポジウム; DNA塩基認識; 銅酵素; 人工変異ヘムタンパク質; 光合成系マンガン錯体; 二酸化炭素固定

The studies on the priority area of "Bioinorganic Chemistry" were carried out for three years with a view to elucidating the structures and specific functions of transition metal ions in biological systems. The following points were intensely pursued :(i) Multiple interactions between biomolecules around the central metal ion(ii) Regulation of biological reaction processes(iii) Precise modeling of biological reactions(iv) Construction of new chemical systemsThe results of the above studies have now been reviewed and summarized to give a clear view on biological reaction mechanisms involving metal ions. They afforded chemical systems which closely mimic the structures and functions of biological metal complexes such as the copper-containing active site of hemocyanin. This research project depended much on the cooperation between scientists from various fields including chemistry, biology, and molecular biology, without which it has not been possible to investigate the metal-containing biological systems at the molecular level. Prior to the 30th International Conference on Coordination Chemistry (ICCC), we held a symposium on bioinorganic chemisrty, in which a number of former and present project team members published their research activities. We prepared a collective report on the whole activities of the research project during the period 1991-1994. The results of the present study will deepen our understanding of the biological functions of metal ions and ultimately of the life phenomena.

“生物无机化学”优先领域的研究历时三年，旨在阐明生物系统中过渡金属离子的结构和特定功能。重点研究了以下几点：（i）围绕中心金属离子的生物分子之间的多重相互作用（ii）生物反应过程的调控（iii）生物反应的精确建模（iv）新化学体系的构建现在对上述研究结果进行了回顾和总结，以对涉及金属离子的生物反应机制给出清晰的认识。他们提供的化学系统非常模仿生物金属络合物的结构和功能，例如血蓝蛋白的含铜活性位点。该研究项目在很大程度上依赖于化学、生物学和分子生物学等各个领域的科学家之间的合作，否则就不可能在分子水平上研究含金属的生物系统。在第30届国际配位化学会议（ICCC）之前，我们举办了一次生物无机化学研讨会，多位前任和现任项目组成员在会上发表了他们的研究活动。我们编写了一份关于 1991 年至 1994 年期间研究项目全部活动的集体报告。本研究的结果将加深我们对金属离子的生物学功能以及最终对生命现象的理解。

项目成果

M. Unno, K. Ishimori, Y. Ishimura and I. Morishima: "High Pressure Flash Photolysis Study of Hemoproteins: Effects of Substrate Analogues on the Recombination of Carbon Monoxide to Cytochrome P450cam." Biochemistry. 33. 9762-9768 (1994)

M. Unno、K. Ishimori、Y. Ishimura 和 I. Morishima：“血红素蛋白的高压闪光光解研究：底物类似物对一氧化碳与细胞色素 P450cam 重组的影响”。

C.Dennison: "Reactivity of Pseudoazurin from Achromobacter cycloclastes with Inorganic Redox Partners and Related NMR and Electrochemical Studies." Inorg.Chem.33. 3299-3305 (1994)

C.Dennison：“来自无色杆菌环碎屑的假天青蛋白与无机氧化还原伙伴的反应性以及相关的核磁共振和电化学研究。”

S.Ozawa: "Spectroscopic Characterization of Peroxo-Iron(III)Chlorin Complexes.The First Model for a Reaction Intermediate of Cytochrome d." Inorg.Chem.33. 306-313 (1994)

S.Ozawa：“过氧化铁 (III) 二氢卟酚复合物的光谱表征。细胞色素 d 反应中间体的第一个模型。”

M.Hisamoto: "Site-Directed Mutagenesis in Hemoglobin:Functional and Structural Study of the Intersubunit Hydrogen Bond of Threonine-38(C3)α at the α1-β2 Interface in Human Hemoglobin." Biochemistry. 32. 13688-13695 (1993)

M.Hisamoto：“血红蛋白定点诱变：人类血红蛋白 α1-β2 界面上苏氨酸-38(C3)α 亚基间氢键的功能和结构研究。”32。13688-13695 (1993)。

T.Ogura: "Time-Resolved Resonance Raman Elucidation of the Pathway for Dioxygen Reduction by Cytochrome c Oxidase." J.Am.Chem.Soc.115. 8527-8536 (1993)

T.Ogura：“时间分辨共振拉曼阐明细胞色素 c 氧化酶还原双氧的途径。”