Cellular respiration and energy analyzer of living cells in high troughput

细胞代谢的复杂调控，也包括活性氧的产生和解毒，在许多生理和病理生理过程中起着核心作用。相关病理包括感染、炎症过程和癌症。为了了解相应的调控过程，有必要在大范围条件下实时监测活细胞的细胞代谢。所提出的用于测定细胞呼吸和活细胞能量状态的高通量分析仪器允许研究细菌，酵母和哺乳动物细胞以及分离线粒体的测量。研究免疫反应和代谢的细胞调节因子将在肿瘤细胞的2D和3D培养中进行，也将在原代免疫细胞中进行。计划对肿瘤治疗中的新活性物质进行研究。此外，还将对新型抗感染药物的代谢作用进行综合表征。它们对细菌和宿主细胞代谢的影响将被研究。此外，计划对细胞呼吸的氧化还原依赖性进行全面的基础研究，重点关注酵母作为重要的模式生物。此外，新的氧化还原活性药物将以其对肿瘤细胞和免疫细胞代谢的影响为特征。

The complex regulation of cell metabolism, which also includes the production and detoxification of reactive oxygen species, plays a central role in numerous physiological and pathophysiological processes. Relevant pathologies include infections, inflammatory processes, and cancer. In order to understand corresponding regulatory processes, it is necessary to monitor cell metabolism in living cells in real time under a wide range of conditions. The proposed analytical instrument for the determination of cellular respiration and the energy status of living cells in high throughput allows the investigation of bacteria, yeast, and mammalian cells as well as the measurement of isolated mitochondria. Work on cellular regulators of the immune response and metabolism will be performed in 2D and 3D cultures of tumor cells, but also in primary immune cells. Investigations are planned with regard to new active substances in tumor therapy. Also new anti-infectious agents will be characterized comprehensively for their metabolic effects. Their effect on the metabolism of both bacteria and host cells will be investigated.Furthermore, comprehensive studies on the fundamental understanding of the redox dependence of cellular respiration are planned, focusing on yeast as an important model organism. Furthermore, novel redox-active agents will be characterized regarding their effect on the metabolism of tumor cells and immune cells.