Cellular respiration and energy analyzer of living cells in high troughput

细胞代谢的复杂调控还包括产生和解毒活性氧，在许多生理和病理生理过程中起着核心作用。相关的病理包括感染、炎症过程和癌症。为了了解相应的调控过程，有必要在广泛的条件下实时监测活细胞中的细胞代谢。建议的分析仪器用于在高通量下确定细胞呼吸和活细胞的能量状态，允许对细菌、酵母和哺乳动物细胞的研究以及对分离的线粒体的测量。有关免疫反应和新陈代谢的细胞调节的工作将在肿瘤细胞的2D和3D培养中进行，但也将在初级免疫细胞中进行。关于肿瘤治疗中的新活性物质的研究正在计划中。此外，还将对新的抗感染药物的新陈代谢影响进行全面的表征。将研究它们对细菌和宿主细胞代谢的影响。此外，计划对细胞呼吸的氧化还原依赖的基础理解进行全面的研究，重点是酵母作为重要的模式生物。此外，还将介绍新型氧化还原活性物质对肿瘤细胞和免疫细胞新陈代谢的影响。

The complex regulation of cell metabolism, which also includes the production and detoxification of reactive oxygen species, plays a central role in numerous physiological and pathophysiological processes. Relevant pathologies include infections, inflammatory processes, and cancer. In order to understand corresponding regulatory processes, it is necessary to monitor cell metabolism in living cells in real time under a wide range of conditions. The proposed analytical instrument for the determination of cellular respiration and the energy status of living cells in high throughput allows the investigation of bacteria, yeast, and mammalian cells as well as the measurement of isolated mitochondria. Work on cellular regulators of the immune response and metabolism will be performed in 2D and 3D cultures of tumor cells, but also in primary immune cells. Investigations are planned with regard to new active substances in tumor therapy. Also new anti-infectious agents will be characterized comprehensively for their metabolic effects. Their effect on the metabolism of both bacteria and host cells will be investigated.Furthermore, comprehensive studies on the fundamental understanding of the redox dependence of cellular respiration are planned, focusing on yeast as an important model organism. Furthermore, novel redox-active agents will be characterized regarding their effect on the metabolism of tumor cells and immune cells.