Cellular respiration and energy analyzer of living cells in high troughput

细胞代谢的复杂调节，其中还包括活性氧的产生和解毒，在许多生理和病理生理过程中起着核心作用。相关病理包括感染、炎症过程和癌症。为了了解相应的调节过程，有必要在广泛的条件下真实的实时监测活细胞中的细胞代谢。所提出的用于测定细胞呼吸和高通量活细胞能量状态的分析仪器允许对细菌、酵母和哺乳动物细胞进行调查，以及测量分离的线粒体。免疫反应和代谢的细胞调节剂的工作将在肿瘤细胞的2D和3D培养物中进行，但也在原代免疫细胞中进行。计划对肿瘤治疗中的新活性物质进行研究。新的抗感染剂也将全面表征其代谢作用。研究它们对细菌和宿主细胞的代谢的影响。此外，还计划以酵母为重要模式生物，对细胞呼吸的氧化还原依赖性的基本认识进行综合研究。此外，新的氧化还原活性剂将表征其对肿瘤细胞和免疫细胞代谢的影响。

The complex regulation of cell metabolism, which also includes the production and detoxification of reactive oxygen species, plays a central role in numerous physiological and pathophysiological processes. Relevant pathologies include infections, inflammatory processes, and cancer. In order to understand corresponding regulatory processes, it is necessary to monitor cell metabolism in living cells in real time under a wide range of conditions. The proposed analytical instrument for the determination of cellular respiration and the energy status of living cells in high throughput allows the investigation of bacteria, yeast, and mammalian cells as well as the measurement of isolated mitochondria. Work on cellular regulators of the immune response and metabolism will be performed in 2D and 3D cultures of tumor cells, but also in primary immune cells. Investigations are planned with regard to new active substances in tumor therapy. Also new anti-infectious agents will be characterized comprehensively for their metabolic effects. Their effect on the metabolism of both bacteria and host cells will be investigated.Furthermore, comprehensive studies on the fundamental understanding of the redox dependence of cellular respiration are planned, focusing on yeast as an important model organism. Furthermore, novel redox-active agents will be characterized regarding their effect on the metabolism of tumor cells and immune cells.