Basic Mechanisms of Genetic Recombination

基因重组的基本机制

• 批准号: 04262103
• 负责人: OGAWA Hideyuki
• 金额: $ 27.26万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04262103/
• 关键词: Genetic recombination; RecA protein; RecA homologues; Rad51 protein; Transposon; Shufflon; Illegitimate recombination; Recombination hot spot; プラスミドシャフロンのDNA逆位; 組み換えホットスッポット; 抗原受容体遺伝子再構成; 相同的組換え; RecA蛋白質; ジャイレース依存の組換え; 遺伝子ターゲッティング; シャフロン

Genetic recombination is a fundamental mechanism by which parental traits of an organism are distributed to offspring. It provides a means of spreading a favorable allele and of eliminating an unfavorable allele in aspecies. Recombination must therefore have playd a crucial role in evolution of the genome. Recombination is also involved in various biological functions in a cell. It participates in repair of DNA damage, a primer formation of DNA replication, production of gene product diversities, alternative gene expression, and chromosome disjunction in meiosis I.Thus the study of recombination is crucial for thge understanding of the mechanisms of biological functions. To further our knowledge of fundamental problems of genetic recombination, a research group was organized supported by this grant. Main distingushed results obtained by this reserach group are as follows.1. The homologues of the recombination gene, recA, were successfully cloned from yeasts, chicken, mouse and human.2. … More A process of resolution of recombination intermediates formed by RecA to recombinant molecules are revealed.3. A recombinaion hot spot specific to mouse female was found and identified at the nucleotide sequence level on the chromosome 17.4. Two novel methods useful for study of genetic recombination were established.(1) A simple and easy method to get various chimera genes between homologues using a recE pathway of E.coli.(2) A comprehensive method to clone a specific site that made a genetic rearrangement from higher eukaryotes was estabilshed using in-gel competitive reassociation method.5. Various kinds of recombination proteins were identified and analyzed : for instance, Dhpl (S.pombe homologue of STPbeta in S.cerevisiae), Lim15 (lily homologues of Dmcl in S.cerevisiae), and RBP-Jkappa(mouse homologue of Su(H) in D.melanogaster).Thus a great advancement was made in analysis of basic recombination mechanism, methodology, and analysis of recombination mechanism in higher eukaryotes by this project. Less

遗传重组是一种基本机制，通过该机制将生物体的父母性状分布到后代。它提供了一种传播有利等位基因并消除不利等位基因的方法。因此，重组必须在基因组的进化中起关键作用。重组也参与细胞中的各种生物学功能。它参与了DNA损伤的修复，DNA复制的底漆形成，基因产物多样性的产生，替代基因表达和染色体分离。重组的研究对于了解生物功能机理的理解至关重要。为了进一步了解遗传重组的基本问题，该赠款的支持。该研究小组获得的主要区别结果如下1。重组基因RECA的同源物成功地从酵母，鸡肉，小鼠和人类克隆了2。 …更多的是RECA形成重组分子的重组中间体的解决过程。3。在染色体17.4的核苷酸序列水平上发现并鉴定出特定于小鼠雌性的重生热点。 （1）使用E.Coli的接收途径在同源物之间获取各种嵌合基因的一种新型方法。鉴定和分析了各种类型的重组蛋白：例如，DHPL（s. cerevisiae中的stpbeta的S. pombe同源物），lim15（s.cerevisiae in cerevisiae in cerevisiae中的DMCL）和rbp-jkap​​pa（rbp-jkap​​pa（sublist）（subbp-jkap​​pa）（sublist of Subilistion in Suclistion Incorminional in D.Melanog aster Inolbistion inter Anally Wasement for Admects a and Adecement act a and Adcanciant a and Actanciant a。通过该项目对较高真核生物的重组机制的方法论和分析。较少的

项目成果

Y.Sekine,N.Eisaki & E.Ohtsubo: "Translational Control Production of Transposase and in Trasposition of Insertion Sequence 1S3." J.Mol.Biol.235. 1406-1420 (1994)

关根永崎

J.Watanabe,K.Nakata,H.Nashimoto,and H.Ikeda.: "Cloning and characterization of a repetitive sequence from Pneumocystis carinii." Rarasitol.Res.78. 23-27 (1992)

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K.Furukawa and Y.Hotta: "_cDNA cloning of a germ cell specific lamin B_3 from mouse spermatocyte and its functional and ectopic expression in somatic culture cells" EMBO J.12. 97-106 (1993)

K.Furukawa 和 Y.Hotta：“_从小鼠精母细胞克隆生殖细胞特异性核纤层蛋白 B_3 及其在体细胞培养细胞中的功能和异位表达”EMBO J.12。

小川智子 篠原彰 小川英行: "真核生物の組換え蛋白質Rad51とRad52の機能と構造" 実験医学(羊土社)「トッピクス」. 12. 527-547 (1994)

小川智子、筱原晃、小川英幸：“真核重组蛋白 Rad51 和 Rad52 的功能和结构”实验医学（Yodosha）“专题”。 12. 527-547（1994）。

小川智子: "真核生物の組換え蛋白質の機能と構造" 第25回 放射性総合医学研究所シンポジウム. 25. 25-37 (1994)

小川智子：“真核重组蛋白的功能和结构”放射线科学研究所第 25 次研讨会 25. 25-37 (1994)。